1、新疆醫(yī)科大學(xué)碩士學(xué)位論文大鼠毛囊干細(xì)胞的分離培養(yǎng)和鑒定方法研究姓名：王祝遷申請學(xué)位級別：碩士專業(yè)：外科學(xué)（泌尿外科學(xué)）指導(dǎo)教師：王玉杰201104新疆醫(yī)科大學(xué)碩士學(xué)位論文2ThestudyofcultivationidentificationonHairFollicleStemcellsoftheratPostgraduate:WangZhuqianSupervis:WangYujieAbstractObjective:Itwaswel

2、lknownthatthestemcellswasdefinitedwithprematurationthepatentofrefreshmentitselfdifferentiationtoembryoadultstemcellsothertissuesgans’cells.Theperiodicitygrowthofhairfolliclerevealedthereareagroupofcellsthatwerenamedafter

3、hairfolliclestemcells(HFSCs)withproliferatingpotentialityinit.Thesourceofhairfolliclestemcellsiswidespreadmeoveritiseasytodrawthematerialsfromtheanimal’sbodythesestemcellsdisplayedpowerfulpotentialofgrowthcolonies’fmatio

4、noutoftheanimal’sbodyasinglecellcanfission1.71038nottoinvolveinthequestionoftheethicsmalitythesestemcellscanbedifferentiatedtoepidermsebaceaehairfollicleneuronadiposecellosteoblastsofth.soHFSCsbecameaidealseedcells.Inpre

5、sentlytherearetwomainmethodsonHFSCs’ionpurificationbymagicactivatedcellsting(MACS)rapidadheringoncollagenⅣ.Theapproachisnotanidealchoicebecauseitsuffersfromexpensiveoperationcomplicatedthestemcellscouldbeinjuriedinthepro

6、cessonlybedoneinbetterconditionlabaty.IncontrasthighlypurifiedHFSCscanbeobtainedbydissectionunderastereocroscopetwostepenzymedigestioncombiningwithrapidadheringoncollagenⅣ.Methods:TheoffspringsofcleaningSDratswasedinthee

7、xperimentAftersterilingtheskinoftherat’sbeardwascutoffthevibrissafolliclesweredissectedunderastereocroscope.theHairfollicleoutersheathwasmovedbyincubatedinDispaseⅡfirstlysecondstepwithamixtureoftrypsinEDTA.Thecellssuspen

8、sionwasculturedin10%fetalbovineserum(FBS)Keratinocytefreeserummedium(KFSM)edbyrapidadheringoncollagenⅣTheculturedcellswerepassagedwhencellswereconfluencewith7080%.edcellsmicrostruturewasobservedunderopticsmicroscopeelect