1、湖北工業(yè)大學碩士學位論文酶法耦合膜技術制備魚鱗膠原蛋白低聚肽的研究姓名：廉志清申請學位級別：碩士專業(yè)：化學工藝指導教師：萬端極李衛(wèi)20100501湖北工業(yè)大學碩士學位論文IIAbstractInthisstudystudyonpreparinghighervalueaddedhighbiologicalactivitycollagenoligopeptidebycomplexenzymehydrolysistechniquemembra

2、netechnologyfromTilapiascalewerecarriedoutThewholeresearehwasaimingatprovidingascientificbasisfeffectiveutilizationoffishscale.InthiswkbasiccomponentsofTilapiascalepretreatmentofscaletheionofcomplexenzymetheoptimizationo

3、fenzymehydrolysisConditiontheionwkacteristicsofmembranetechnologyofvacuumfreezedryingwereinvestigated.Themainresearchresultswereasfollows:1.Thedecalcifyingdegreasingprocessoffishscalewerestudied.Theconcentrationofacidalk

4、alitimeofprocessingthesolidliquidratiosignificantlyinfluencedtheeffectofpretreatment.Theresultsshowthat:decalcificationratereachedabove98%when3%hydrochlicacidwasusedwith1:15solidliquidratiof4hwhen8%SodiumHydroxidewasused

5、with1:15solidliquidratiof3hthefatcontentdecreasedto0.16%～0.22%thecollagencontentcanincreaseto93.6%～94.1%.2.Theextractiontechnologyofscalecollagenoligopeptidewasinvestigated.Thecomplexenzymeconditionweredetermined.Theresu

6、ltsshowedtheoptimalconditionofenzymehydrolysiswasusing2%animalproteaseneutralproteasewithaproptionof1:1.2ratioofsolidliquid1:8temperaturebetween58～60℃pH=6.8～7.0extractiontime4hours.Theconversionofcollagenoligopeptidereac

7、hed94%~95%thecollagenoligopeptideofcontentis86.6%～88.2%.3.Theimpurityremovalconcentrationtechnologyofenzymesolutionwasresearcheddiscussed.Theenzymesolutionwasdonebytheredermembranetechnologywhichwere100nmMFMWCO2500DalUFM

8、WCO300~500NF.thepurityofcollagenoligopeptideraisedto96%thecollagenoligopeptidewithmolecularweightbetween300~1000Dalcontentincreasedtoabove95%qualityofoligopeptidewasgreatlyimproved.4.Vacuumfreezedryingtechnologyofconcent

9、ratedsolutionwasstudied.Theanalysisoftheexperimentalresultsshowedthattheoptimumtechnicalconditionsofthefreezedryingasfollow：heatingtemperatureofshellwas65℃thematerialthicknesswas12mmthehothousepressurewas50Pa，freeze–dryi