1、合肥工業(yè)大學(xué)碩士學(xué)位論文一株脂肽生物表面活性劑產(chǎn)生菌篩選及其脂肽性質(zhì)研究姓名：錢曉勇申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：農(nóng)產(chǎn)品加工與貯藏指導(dǎo)教師：葉明劉國慶20090401StudiesonthescreeningofalipopeptidebiosurfactantProducingbacterium一‘l‘a(chǎn)ndpropertiesofthelipopeptideABSTRACTThispaperstudiedthescreeningofabi

2、osurfactant，thepropertiesandthesafetyevaluationforapplicationThemainresultswereasfollows：1AseriesofbacteriawereisolatedfromsoilsamplesandwatersamplesfromHefeiUniversityofTechnology，HefeioilstoreroomandDongyingVictoryscum

3、poolfromShangdongprovincebyaseriesofstepsincludinghydrocarbonenrichmentculture，hemolyticactivityassayonbloodagarplatesandsurfaceactivityassayetcOnestrainwaspurifiedandwasnamedasH02ItisidentifiedasaBacillussubtilisbybioph

4、ysicalandbiochemicalexperimentsaccordingto((Bergey’SManualofDeterminativeBacteriology)，16SrDNAsequenceanalysisThebiosurfactantproducedbystrainH02wasfoundedasalipopeptidebiosurfactantIthasasatisfactingactivitywithaoildisp

5、lacementdiameterat92cmFurthermoretheemulsionofthislipopeptideisfineaswellformaintaintheemulsionforatleastl0days2TheconditionforlipopeptideproductionwasoptimizedbysinglefactorexperimentsandorthogonaltestResultswereasfollo

6、ws(g／100mL)：solublestarch25，NH4N0306，MgS047H20O04，CaCl20008，yeastextractpowderO08，KH2P04O24，Na2HP04096，pH7075Attheinoculumof5O％，under35℃，theyieldoflipopeptidewas318g／L3Thephysicalandchemicalpropertiesoflipopeptideproduce

7、dbystrainH_02wasinvestigatedResultsshowedthatthisbiosurfactproducedbyH一02hasaexcellentemulsionandvesicantcharacteristic，andanti—microorganismstraitsjustlikeantibioticsSoitcouldbeusedformanydomainsasMEOR，F(xiàn)oodandPharmacyAl

8、sothislipopeptidecanmaintainitsactivityunderserialextremeconditionslikehightemperature，highconcentrationsalt，especiallyCa2十cation，andlowpHThesuitablepHwasidentifiedbetween40and1004。Theacutetoxicitytestandthirtydaysfeedin