1、東南大學(xué)碩士學(xué)位論文NGF對(duì)新生腦神經(jīng)元細(xì)胞缺氧缺血損傷保護(hù)作用的研究—NGF對(duì)線粒體結(jié)構(gòu)及功能的影響姓名：黃莉申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：兒科指導(dǎo)教師：蔣犁20040508ABSTRACTAbstractTitle：TheProtectiveEffectofNGFonHypoxic—ischemicNeuronalCellsiUNeonatalBrain——toExploretheEfkctofNGFonTheirMitochondria

2、lStructureandFunctionPostgraduate：HUANGLiTutor：JIANGLiUniversity：SoutheastUhiversity0bjective：TodeterminetheprotectiveeffectofNGFonhypoxic—ischemic(HI)neuronalcellsofneonatalbraininvitroMethods：(1)HImodelofneuronalcellso

3、fneonatalbraininvitrowasinducedbythedeprivationofoxygenandglucoseaccordingtotheimprovedGoldberg’smethod②Inordertofindouttheoptimalconcentration，MTTmethodwasappliedtomeasuringthevitalityofneuronalcellstreatedwimNGFindiffe

4、rentconcentrationofNGETheresultwascomparedwimthevitalit、rofneuronalcellsinthenormalgroup，andinthecontrol(H11oneatdifierenttimepoints(0、24hoursafterHI)⑨Neuronalcellsinvitroweredividedinto3groups：thenormal、thecontrol(HI)、a

5、ndthetherapeuticgroupThemorphologicchangesofneuronalcellswereobservedbylightandelectronicmicroscopes。TheexpressionValHeofCyt—CandA1FmRNAofdifferentgroupweredeterminedbyimmunocytochemistryandRrrPCRrespectivelyResults：(1)T

6、hevalueofMTTinHIgroupwassignificantlylowerthanthatinthenormalgroupandthebesttherapeauticoneAccordingtothevalueofMTT80ng／mIofNGFiStheoptimalconcentration(④Comparedwiththatofnormalgroupandthetherapeuticone，thevalueofCvtCan

7、dAIFmRNAofthecontr01wassignificantlyhigher伊O05)Therewasriosignificantdifierencebetweenthenorlllalgroupandthetherapeuticone(9“曲tandelectron—microscopicobservationshowedthattheapoptosisandtheabnormalmorphologicchangesofneu

8、ronalcellswereobviousinthetherapeauticgroup@NGFwhichwasusedat240hourafterHIcontrolgroup，comparedwiththoseinthehoursafterH1waslesseffectivethanthatatConclusion：InvitroHImodelofneuronalcellsofneonatalbrainCansimulateHIbrai

9、ndamage(HIBD)ExogenousNGF(80ng／m1)hasthebestprotectiveeffectonHIneuronalcellsTheearlierNGFwasusedthebeRertheresultwasKeywords：Neuronalcells；Hypoxiaischemia；Nervegrowthfactor(NGt)Apoptosis—inducingfactors(AIF)；Cytochromec