1、揚(yáng)州大學(xué)碩士學(xué)位論文灰葡萄孢毒素的產(chǎn)生條件及其對(duì)番茄植株的致病作用姓名：魏伶俐申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：植物病理學(xué)指導(dǎo)教師：童蘊(yùn)慧20070501魏伶俐灰葡萄孢毒素的產(chǎn)生條件及其對(duì)番茄植株的致病作用ProductionofBotrytiscinematoxinandpathogeniceffectsontomatoplantABSTRACTToxinWasakindofsecondarymetabolitesofplantpathogen

2、sIthadimportanteffectincourseofdiseasesbecausepathogenscouldnsetoxin舔chemicalweaponattackhostplantWehadfeltthatstudyonthetoxincouldleadtoagreaterunderstandingofthepathogenicfactorsofpathogens，pathologicalalterationofhost

3、plantsincellstructure，andpathogenicmechanismsofpathogensBorrytiscinemawasakindofimportantpmhogenofplantandcouldproducetoxin(BCtoxin)inliquidcultureThecrudeBCtoxinwasobtainedfromtheculturedfiltratewhenitwasextractedwithch

4、loroformandthenchloroforminextractedproduceWasevaporatedThebiologicalactivityassayofthecrudetoxinshowedthatitcouldinducethesimilarsymptomoftomatoleafgreymould，andinhibitthegrowthoftomatoradicleandmaketheseedlingwiltingAc

5、cordingtotheaboveresultsweconcludedthatBCtoxinhadtoxicitytotomatoplantandseedThecultureconditionfortheproductionofBotrytiscinematoxinWasstudiedItWasappropriateforthetoxinproduction硒follows：TheoptimummediumwasimprovedFrie

6、sIIlmediuminwhichpHvalueWaS6ThestrainsWasculturedfor2025daysat2225℃，withdarknessandnoshakeThecrudeBCtoxinobviouslydamagedthecellmembraneoftomatoleafThepermeabilityofcellmembranesWaschangedandtheelectrolytesinthecellsoutf

7、lowedwhentomatOleavesweretreatedbythecrudetoxinWhentomatoleaveswBfetreatedbythecrudetoxindiluted50times，thedamagemteoftoxintocellmembraneswasabove90％ThoughthecrudetoxinWasdiluted200timesthedamageratetocellmembraneoftomat