1、廣州醫(yī)學(xué)院碩士學(xué)位論文地塞米松對嘌呤霉素?fù)p傷小鼠腎小球足細(xì)胞系保護(hù)作用的分子機(jī)制研究姓名：溫躍強(qiáng)申請學(xué)位級別：碩士專業(yè)：兒科學(xué)指導(dǎo)教師：于力20090520廣州醫(yī)學(xué)院碩十學(xué)位論文地塞米松對嘌呤霉索損傷小鼠腎小球足細(xì)胞系保護(hù)作用的分子機(jī)制研究ABSTRACTObjectireInordertodemonstratethepossibleanti—proteinuriamechanismsofdexamethasone，astudywasd

2、esignedtoexplorethepodocyteapoptosisrateandexpressionanddistributionofpodocinandCD2APinatimeseriesscaleinthecaseofpodocyteinjuryandprotectioninvitroMethodsMousepodocytecellline(MPC5)wereculturedin002％DMSOincontrolgroup，s

3、ubjectedtopuromycinaminonucleoside(PAN)treatmentaloneorwithdexamethasone(DEX)inothertwogroupsThepodocytemorph0109ywasobservedandtookpicturesbyphase～contrastmicroscope，thenthedifferencesofmorphologyandareasbetweeneachgrou

4、pswereanalyzedbyImageJTheapoptosiSratewasdetectedbyflowcytometry，thedistribution，mRNAexpressionandproteinexpressionlevelofpodocinandCD2APweredetectedbyindirectimmunocyto—fluorescence，semi—quantitativeRT—PCRandWesternblot

5、，respectivelyResults(1)Thewel1一developedpodocytearborizationandinterconnectionwasformedincontrolgroup，butPANtreatmentledtosignificantshrinkageofpodocytes(PO01)，togetherwithpodocytefootprocesseretraction，effacementandloss

6、ofcellcontactTheapoptosiSratewasincreasedat48h(PO05)RT—PCRfoundpodocinandCD2APmRNAexpressionpronetodecrease，WesternblotshowedpodocinproteinexpressionsignificantdecreaseandimmunocytochemistrysuggestedpodocinandCD2APdisapp

7、earedinthecellularmembraneafterPANtreatment(2)DEXsignificantlypreventedtheshrinkageandapoptosisofpodcyte，increasedpodocinandCD2APmRNAexpression，andincreasedpodocinproteinexpressionlevelat48h(P005)Theabnormaldistriutionof