1、大連理工大學(xué)碩士學(xué)位論文光鑷的光壓力測(cè)量及光鑷與膜片鉗結(jié)合的研究姓名：韓雪申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：光學(xué)指導(dǎo)教師：潘石20090601光鑷的光壓力測(cè)量及光鑷與膜片鉗結(jié)合的研究Themeasurementofopticalforcefromopticaltweezersandtheooo‘‘n‘’o‘research0nthecombinationolopticaltweezersandpatchclampAbstractBasedonth

2、eintroductionofopticaltweezers，themainpointsofthisdissertationarethemeasurementofopticalforcefromopticaltweezerswithdifferentmicroscopeobjectives，andtheinitialresearchofcombinationofopticaltweezersandpatchclampaimingtese

3、archcell’Selectricalcharacteristics強(qiáng)建firstchapteristheintroductionofopticaltweezers’historymaintheorycharacteristics，newdevelopments，applicationandthecombinationofopticaltweezersandotherapproachesusedinresearchThesecondc

4、hapteristheintroductionofmajoropticalelementsofsinglebeamgradientforcelaseropticaltrapandthedifferentmethodsofpositiondetectionandforcemeasurementThethirdchapteristhemeasurementresultsofopticaltrappingforceusingLOCIoptic

5、altweezers—producedbyShanghaiUSTCLOTTechnologyCO——basedonthetheoryofthermalequilibriumThroughthecomparisonoftheresultsfromusing40Xmicroscopeobjective謝餓O65numericalapertureandthe100Xmicroscopeobjectivewith125numericalaper

6、ture，itshowsthatIOOXmicroscopeobjectivehavingbiggeropticalforcenotonlyinlateraldirectionbutalsoinaxialdirectionInthesameexperimentconditionlikeincidentlaserpowertrappedeel]etc，thestiffnesscoefficientusing100Xmicroscopeob

7、jectiveishigherthanthatof40Xmicroscopeobjective，nlehighertrappingabilityof100Xmicroscopeobjectiveshowsintheaspectsofthetrappingefficiencyishigherandtheabili夠testricttrappedcell’Smovementintheaxialdirectionandthefluencyof

8、manipulationofthetrappedcellAccordingtothisresult，microscopeobjectivewithhigheramplificationandnumericalapertureshouldbeusedinopticaltrappingresearchforthepurposeofhighertrappingefficiencyandfluentmanipulationThefonllcha

9、pteristheinitialresearchofthecombinationofLOTIImodelopticaltweezersandEPC一10modelpatchclamp?；aimofthiscombinationistoimprovetheresearchofthemeasurementofelectricalcharacteristicsofneuroncellsnlrou醢theexperimentprocess，a

10、fteropticaltweezerstrappingacell，themicropipetteofpatchclampapproachesthecellandpushesthecell，butmaintainsthecellstillintheopticaltrappingwellUsethestiffnesstoemcienttocalculatetheforcecarriedoutbythemicropipetteTheresul