1、南開(kāi)大學(xué)碩士學(xué)位論文磷（膦）酸肌醇化合物庫(kù)的篩選及生物學(xué)功能的研究姓名：張晶申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：李魯遠(yuǎn)201205AbstractLun2cancerisoneofthemmncausesofcancerrelateddeathintheworldAbout7585％oflungcanceriscomposedofNonsmallcelllungcancer(NSCLC)Peoplehavemadeal

2、otofprogressesincancerdiagnosis，chemotherapyandradiotherapybutthepatientsofNSCLCmayshowresistancetoradiotherapyandchemotherapyinthesurgicaltreatmentoflatestageTheoverall5yearsurvivalrateofDatientsofterminalstagesofcancer

3、afteroperationremainslessthan10％WeneedtofindnewmethodsteducetheincidenceP13K／AktpathwaywasfoundtobeimportanttoNSCLC，whictlCallbethetherapeutictargetforanticancerdrugsInosit01DhosphatecompoundscanbedividedintoPhosphoinosi

4、tide(IP)andPhosphatidylinositolphosphate(PtlnsP)，andplayanimportantroleincellsignaltransductionassignaling：moleculesXiprofessor’Steamhavesynthesizedsomeinositolcompoundswhichcanimprovethecellpenetrating，phosphorothioatea

5、ndinositolcompoundsofphosphoInicacidtoimprovethestabilityofthephospholipase，andfinallybuildthephosp]hoinositidephosphonicacidinositolanalogⅣescompoundlibrarytogetherwithsomeWedosomescreeningresearchusingA549cell，includin

6、gprescreeningwiththeconcentrationoflOItg／mLofthecompoundsandanalysisoftheconcentrationgradientfor14compoundswithbetterinhibiteffect，anddeterminetheirIC50Combinationofthe14compoundsandCisplatinwasinvestigatedtooWealsostud

7、ytheireffectonMCF10Aandother6cancercelllines，suchasMCF7Usingwesternblottingwedosomeresem：chtodeterminetheireffecttoP13K，Akt，Erkandotherkinases0809，0812，0414一MnwereinjectedtoC57micetodetectwhethertheycaninhibitthegrowthof