1、摘要摘要目的:本實驗通過連續(xù)記錄大鼠局灶性腦缺血再灌注不同時間段的動脈血壓(arterialbloodpressureABP)、心電圖(electrocardio鄉(xiāng)amECG)信號、心率(heartrateHR)，以及腎交感神經(jīng)活動(RenalSympatheticNerveActivityRSNA)并分別進行處理和分析，探討急性局灶性腦缺血后自主神經(jīng)功能障礙對心血管系統(tǒng)的影響。方法:采用線栓法制作大鼠局灶性腦缺血再灌注模型，將30只成

2、年雄性SD大鼠隨機分為缺血組(ischemian=24)及假手術(shù)組(shamoperationn=6).缺血組再根據(jù)缺血時間不同和是否再灌注又分為4個組，分別為:缺血1小時組(ischemiaforlhour11)、缺血3小時組(ischemiafor3hours13)、缺血5小時組(ischemiafor5hours15)和缺血3小時再灌注2小時組(ischemiafor3hoursandreperfusedfor2hours1382

3、)，每組6只大鼠。通過PowerLab8spSCSIUSB記錄儀連續(xù)采集ABPHRECG和RSNA信號。從記錄的ABPHRECG以及RSNA時間序列中，以每2分鐘為一個時間單位，利用Chart4軟件獲得平均動脈壓、心率、心率變異性序列及腎交感神經(jīng)活動。對經(jīng)過等間距處理后的心率變異性序列進行功率譜分析。對實驗組的所有數(shù)據(jù)進行配對t檢驗。結(jié)果:1MABP與HR:缺血組與假手術(shù)組相比，缺血后均顯著升高MO.05)缺血后30分鐘MABP達到峰值

4、，而缺血2.5小時HR到達高峰并且維持明顯高于缺血前的水平再灌注后MABP與HR均有不同程度的降低，MABP比缺血前水平低而HR比缺血前水平高。2HRV:急性腦缺血后，總功率譜(totalpowerTP)、低頻段功率(lowfrequencypowerLF)和高頻段功率(highfrequencypowerIIF)均明顯降低(P0.05)，中頻段功率(mediumfrequencypowerMF)及MFHF比值均明顯升高(P0.05)，

5、缺血3小時以后，各項HRV頻域分析指標均明顯下降(低于基AbstractAbstractObjectives:ToinvestigatethechangesinautonomicnervoussystemcausedbyfocalcerebralischemiainSDrats.Arterialbloodpressure(ABP)electrocardiogram(ECG)heartrate(HR)andrenalsympathetic

6、nerveactivity(RSNA)wererecordeddealtwithandanalysed.Methods:FocalcerebralischemiareperfusioninSDratswasinducedbyintraluminalocclusionofmiddlecerebralartery.Ratswererandomlydividedintothefollowinggroups:pseudosurgical11(i

7、schemiafor1hour)13(ischemiafor3hours)15(ischemiafor5hours)and1382(ischemiafor3hoursandreperfusion.(for2hour.Therewere6ratsforeachgroup.Ratsinexperimentalgroupsweresuferedfocalbrainischemiareperfusionthroughmiddlecerebral

8、arteryocclusionbythenylonthread.ABPECGSHRandRSNAwerecollectedfordisplayingand.lateranalyzing勿PowerLab8spSCSIUSBdataacquisitionsystem(ADInstruments).UsingthesoftwarenamedChart4meanarterialbloodpressure(IvIABP)meanheartrat

9、e(HR)andsequenceoftheinterrelateddataofheartratevariability(HRV)wereobtainedfromthetimeseriesin2minutesperiod.TheHRVsequenceswereequalizedandprocessedbypowerspectralanalysisthroughtheFastFourierTransform(FFT).Pairedttest

10、swereperformedforalldataofgroups.Afteraprocessorfortissuemanagethebrainswerecorona)sectionedat30tmbyarotarymicrotomeandstainedwithHematoxylinandEosintoobservethemorphologicalalterationofneuroninischemicarea.Theotherbrain

11、swerecorona)sectioneddirectlyat2mmandstainedwithTTCtoobservethealterationinischemiaarea.Results:1.MABPandHRinsurgicalgroupswereincreasedafterischemiavscontrol.MABPreachedthehighestlevelinhalfanhourafterocclusionandHRreac

12、heditsclimaxin2.5hoursafterischemiamaintainedatahigherlevelthanthatofthepreischemia.AfterreperfusionMABPandHRweredecreasedtodiferentdegrees.MABPwasrecoveredtothepreischemialevelbutHRwaschangedtoalittlebithigherthanthat一I