1、中南大學碩士學位論文妊高征胎盤絨毛組織內(nèi)皮素1及其受體的mRNA表達姓名：王康申請學位級別：碩士專業(yè)：婦產(chǎn)科學指導教師：萬亞軍20040520碩士學位論文摘要ExpressionofendothelinIandendothelinreceptorsmRNAinplacentalvillousfrompregnancyinducedhypertensionABSTRACTobjective：TodetectthemRNAexpressio

2、nofendothelinI(ET1)，ETlreceptorsE1’AandETBinplacentavillousfromnormalpregnancy(ro)andpregnancyinducedhypertension(PIH)Andtoinvestigatetheroleofendothelin1andendothelinreceptorsinthepathogenesisofpregnancyinducedhypertens

3、ionMethods：ThemRNAlevelsofET1，ETAandETBweredeterminedusingRTPCRandexpressedasarbitraryunitsaftercorrectionforcontrolGAPDHgenemRNAlevelsinplacentavillousfrom30NPand60PmResults：ThemRNAexpressionofET1，ETAandETBwerefoundinpl

4、acentafrombethPIHandNPThemRNAlevelsofET1andETAweresignificantlyhigherinPIHthanthatinNP(po05)，whiletheETBmRNAlevelsweresimilar(p)005)Conclusions：1ThernRNAexpressionofE，r1。E1AandETBwerefoundinplacentaTheplacentaisshortofne

5、rveregulationitselfSOweconsiderETasaparacrinefactorplaysallimportantroleinfetalplacentalcirculationETETRsystemmayparticipateinthenormaldevelopmentofplacentaandtheregulationofthefetal—placentMbloodvessels2肌lenET一1iscombin

6、edwithETAitcanaroseintensevesselcontraction。BecausetheexpressionofETIandETAmRNAwerebothsignificantlyhigherinplacentaofPIH，theresistanceoffetalplacentalcirculationwasincreasedand也eplacentawasshortofbloodandoxygen，andthepl