1、東南大學(xué)碩士學(xué)位論文對培美曲塞耐藥的胰腺癌Patu8988細胞中CYP1B1的表達及粘附增殖行為的檢測姓名：黃聰申請學(xué)位級別：碩士專業(yè)：臨床醫(yī)學(xué)（外科學(xué)）；消化道腫瘤基礎(chǔ)與臨床指導(dǎo)教師：石欣20110602Abst心屺tTheexpressionofCYP1B1andtheadhesive，pr01iferatiVecapacitlyofPemetrexed—resistantPatu8988pancreaticcancercellli

2、neGraduate：HuangCongSuperVisor：ShiXinMedicalcollegeofSoutheaStUniVersityAbstractobjectiVeToinVestigatetheexpressionofCYPlB1inPemetrexed—resistantPatu8988pancreaticcallcercellline，aJldevaluateitse日ectontheadhesionandproli

3、ferationbehaViorsofpancreaticcallcercell1ineMethodsTheexpressionofCYP1B1inPemetrexedresistantpallcreaticcancercelllineandparentalcelllineweredetectedbyRT—PCRandWestemblotanalysis，respectiVelyThedistinctionsofadhesionandp

4、roliferationbeha“orsbetweenPemetrexedresistantpancreaticcallcercelllinealldparentalcelllineweredetectedbyadhesionassayandMTTaSsayResultCompared州thparentalcellline，CYP1BlinPemetrexed—resistalltcenlinewereup—regulatedby52％

5、andby66％weredeteminedbyRT—PCRandWestemblot，respectiVely(尸005)AdhesionassayshowedthatthecapabilitiesofadhesionofPemetrexed—resistaIltpancreaticcancercellsigni矗cantlyemlancedbylO％thanthatofparentalone(尸005)Andthecapability

6、ofproliferationinPemetrexedresis伽1tpaJlcreaticcallcerceUlinewaSweakerthanthatofparentaloneConclusionCYP1B1enhancedobViouslyinPemenexed—resistantpancreaticcaIlcercelllinePatlJ8988Comparedwithparentalcellline，Pemetrcxedres