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1、山西醫(yī)科大學(xué)碩士學(xué)位論文變應(yīng)性鼻炎輔助性T細(xì)胞TIM1的表達(dá)姓名：武文魁申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：耳鼻咽喉頭頸外科學(xué)指導(dǎo)教師：趙長(zhǎng)青20080331山西醫(yī)科大學(xué)碩士學(xué)位論文InvestigationintoTIM一1expressionofhelperTlymphocyteinallergic●●●●●rhinitisDatientsABSTRACTObjectiveToevaluatethesignificanceofTcellimmu

2、noglobulindomainandmucindomain1(TIM1)expressioninHelperTlymphocyteinallergicrhinitis(AR)patientsTIM1hasbeenidentifiedasbeingencodedbyallimportantatopysusceptibilitygeneMethodsHumanpefipheraibloodmononucleareells(PBMC)wer

3、eseparatedbydensitygradientfrom20healthyhumandonorSand20ARsufferersTheMagneticcellsortingtechnique(MACS)wasappliedtopunfyCD4TcellsandCD4十CD45RATcellsImmunofluorescencestainingandflowcytometrywereusedtodetecttheHelperTlym

4、phocytemarkerTIM—l；TIM一1positiveHelperTlymphocytesfromARsufferersandhealthyhumandonomweretestedbyinfusingPHA(20ug／m1)intotheculturemediumDCWasconditionedincultureofDermatophagoidespteronyssinus(50000SBU／m1)orLPS擔(dān)Ong／m1)i

5、n，vitro，respectivelyThenCD4十CD45RATcellswerecoculturedwiththeDermatophagoidespteronyssinusorLPSconditionedDCAtthesanletimeTIM1positiveHelperTlymphocyteswerecountedEnzymelinkedimmunosorbentassay(ELISA)wasusedtoanalyzeIL4a

6、ndIFN吖fromsupernatantsoftheseculturesforindirectlyreferringtotheproportionofn12廠I’111ResultsTIM—lpositiveCD4Tcellsinallergicrhinitiswere948％士151％，higherthanthatinhealthycontrols(312％032％)(PO05)，TIM—lpositiveCD4十CD45RATce

7、llsincreasedafterinfusingPHAintotheculturemediumTIM1positiveCD4CD45RATcellsinDermatophagoidespteronyssinusgroupswere1081％4148％，higherthanthatinLPSgroups(607％4145％)(P005)IL4andIFN吖levelsinDermatophagoidespteronyssinuswere

8、74611382pg／ml；2531723pg／ml，respectively；andinLPSgroupswere2857士336pg／ml；163418237pg／m1ConclusionTIM1positiveHelperTlymphocytesinARsuffererswerehigherthanthatinhealthyhumandonorsDCpresentantigentoHelperTlymphocytesandincr

9、easedexpressionofTIM一1onHelperTlymphocytes；TIM—lpositivenaiveTcellswereelevatedafterinfusingEuropeanstandardallergenintotheculturemediumandthusdrovednafveCD4TcellstodifferentiateintoTh2cells，suggestingthatTIMlexpressiono

10、nHelperTlymphocytesmightbebeneficialindrivingnafveCD4TcellstodifferentiateintoTh2cells，andthusinducingTh2cytokinebiasedimmuneresponses1KeywordslRhinitis；Allergic，HelperTlymphocyte，Tcellimmunoglobulindomainandmucindomain—

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