1、河南農(nóng)業(yè)大學(xué)碩士學(xué)位論文雞志賀氏菌ESBLs的基因型檢測及其感染的實(shí)驗(yàn)性治療姓名：司紅彬申請學(xué)位級別：碩士專業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：胡功政20060601DetectionofESBLsgenetypeproducedbyshigeilafromchickenandtentativetreatmentsofitsinfectionSupervisor：ProfHuGongzhengMasterCandidate：SiHong—binAB

2、STRACTMICofcommonantibioticsagainstshigellaisolatedfromchickenweredeterminedbytheameliorativetwofolddilutionmethod，theESBLsproducedbyshigellaweredetectedbydoubleslipssynergyanditsphenotypeswerealsomadesure、析tIlPCRinthisp

3、aperThetreatmentresultsofcompoundsuspensionpreparationofceftiofurwhichweretentativelymadebyourlaboratoryagainstdiseasesinfectedbyshigellaproducedESBLswereobserved，inordertounderstandthecurrentsituationandmechanismofdrugs

4、’resistanceandinvestigatedmoleculemechanismofitadrugs’resistancetheoriesbasedontheexperimentwereofferedforclinicaltreatments，newthoughtsonexploitationofnewveterinarydrugswereofferedatthesaDietime111eresultsofthedetection

5、ofBLAandESBLsshowedthatshigellaclinicalstrainsisolatedfromchichenhadproducedBLAbynitrocefindisctest，butnoneofAmpCESBLswasfoundbydouble—discsynergymethod11leprimerWasdesignedfortheESBLsproducedbyshigellaandamplificationby

6、PCRthencloningandsequencing，ananalysiswasexecutedbycontrastingwiththesequenceofU48775塒吐lasoftwarecalledDnastarMegAlignSequence，thehomologygene、7lritll992％wasidentitiedtoTEM1(U48775)，theresultrevealedthe287aminoacidprotei

7、ndifferingbythreeaminoacidsubstitutionsfromplactamaseTEM—l(Asp33GluThrl86砧如Ar9271Gln)，ItharboredaTEM—ll6哆pegene謝tll998％identitytoAY903309(TEM一116)，AminoaciddifferencesincomparisontotheaminoacidsequenceofESBLsTEMl16withDn

8、astarMegAlignSequenceDistanceswerefounddifferingonlyone(Ser51Glu)，althoughatthepoint403TchangedintoC，butitshouldbesilentmutationandnotleadtotheaminoacidchangementThegenetypeofenzymeWascalledTEMlVtemporarilybecausetherewe