1、青島大學(xué)碩士學(xué)位論文擬缺血再灌注損傷對(duì)大鼠海馬神經(jīng)元NMDA受體亞單位的影響及人參皂苷Rg的干預(yù)姓名：房純正申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生理學(xué)指導(dǎo)教師：金毅20060601EFFECTSoFMODELINGISC皿MI(ALREPERFUSIoNINⅢRYONNMDARECE盯ORSUBUNrrSINCUUTUREDRATHIPPOCAMPAINEURONSANDTHEINTERVENTION0FGINSENOSmER92AbstractO

2、biectiveTbmakeanischemicalreperfllsioniniurymodelinculturcdrathippocampalneumnsandobservetheexpressionofNMDArecepIorsubunitsNRl，NR2Bandglutamateofrathjppocampalneuronsafterischemicalreperfusioninjury24h，48hand72hTbstIIdy

3、thepatllogenesyofischemicalf印erfusioninjuryandlheef：fcctsofginsenosjdeR＆oniIljuredneuronsonfheaspectofexcilotoxicityofexcitatoryaminoacidespeciallytheexpressionofreceptorsubunitSMethodsTbmakeaIlischemicalreperfusioninjur

4、ymodelbyoxygenand酉ucosedeprivation(0GD)inculturedrathippocampalneuronsNissl’sstajningwasusedtoidentifyhippocampalneuronsTheiIljuryofneuIonswasdetecIedbytheMTTI℃ductionassaymechodanerOGDT1leexprcssionofreceptorsubunjtsNRl

5、，NR2Band出utamatewasdetectedbyimmunocytochemicaltechniqueLaserscallningconfocalmicroscopeandFluo3／AMwereusedtomeasuretheintmcellularcaIciumnuorescencepixelvaluesofhippocampalneuronsResultsTheactivityofneuronswasdecreaSedi

6、nmodelgroupthanincontml日oup俾005)ThelongerwasthereperfIlsjontimethelowerwastheactivityofneuronSThercIeaseofglutamatefromdamagedneumnswasincfeasedsignjf：icantlycomparingwithcontmlgroup(P(005)Theexprcssionofrcceptorsubunits

7、NRlwasincreasedandlhatofreceptorsub咖itsNR2BwasdecrcaSedcomparingwjththeconlr01gmup(P005)The日uorescencepixelvalueofiIltracellularfrcecalciumofhjppocampalneuronswassignificantlyhigllerafterOGD(P005)Theactivityofneuronswasi

8、ncrcasedsignjficantlyinhippoc鋤palneuronstI℃atedbyginsenosideR92(PO05)Thereleaseofglutamatefromneuronstreatedby百nsenosideR＆wasdecrcasedsignificaIlnycompa血gwiIhthemodelgmup@O05)ne【c一】iandtheexpressionofNRldecreasedandtheex

9、pressiOnofNR2BincreasedcoInparjngwiththemodel掣oup(P005)ConclusionsTherclcaseofmutaⅡlateincreasedwhichactivatedmeexcitatory鋤inoacidreceptorsexcessivelyinischemicalreperfIlsionⅫuredhippoc鋤palneuronsT1leexpressionofreceptor

10、subunitsNRlwasiIlcreasedandthat0freceptorsubunitsNR2Bwasdecreased，wllichinducedtIlefLlnctiOnchangeOfthereceptorsaIldresulted協(xié)the【ca2】ioverIoadingafterthej11juryAHthisinducedtheilljuryoftheneuIDnscausedbyaserlesofbiochemi

11、caleventdependingC礦intra—cenlllarGinsenosideR盤playsitsprotectjveroIeininllibitingthereleaseofglut鋤ate，regulaIingtheexpressiOnofexdtatOryaminoacidreceptorSsubunits，reducingtheinnuxofcalciumion，andincreasingtheaciVityofneu