1、中南大學(xué)碩士學(xué)位論文大鼠視神經(jīng)切斷后視網(wǎng)膜雙極細(xì)胞上PKC—alpha和recoverin的表達(dá)變化姓名：蔡艷申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：神經(jīng)生物學(xué)指導(dǎo)教師：羅學(xué)港20050501碩士學(xué)位論文摘要ChangesofexpressionofPKCalphaandrecoverinintheretinalbipolarcellsofadultratsaftertheopticnervetranseetedABSTRACTItiswellknow

2、nthatthereexistsantegradedegenerationafteropticnerveinjtuy[toweverrecentstudieshavedemonstratedthatinvisualnervesystem，inadditiontoantegradedegenerationtherealsoexiststransnearaldegenerationButtoourknowledgetheinformatio

3、naboutofretinalbipolarcellsisnotavailableuntillnowInthisstudywetestedthehypothesisthatretinalbipolarcellsareundergoingchangesafteropticnervetransectionFortyoneadultSDratswererandomlydividedinto3groups：normalcontrol(n=5)，

4、shamoperation(n26)andexperimentalgroup(opticnervetransected，n=30)Theanimalswereallowedtosurvivefor3，5，7，14，21and28daysthensacrifiedrespectivelynleretinaeswereremovedanddetectedbyimmunohistochemistryandimmunofluorescenceu

5、singspecificantibodiesagainstPKCalphaandrecoverinWefoundthat①I(mǎi)nnormalretinaPKCalphapositiveproductsweremainlylocalizedintheinnernuclearlayer(INL)，innerplexiformlayer(IPL)andganglioncelllayer(GCL)，especiallyintheIPL，Axons

6、andexpandedterminalsofbipolarcellswereclearlyshown②Comparedwiththenormalcontrol，thenumberofpositiveterminalbuttonsintheIPLwasgraduallyincreasedfrom3days，7daysto14days，followedbyadecreaseatthe21stday③Innormalretinarecover

7、inpositiveproductsweremainlylocalizedinouternuclearlayer(ONL)，INLandGCLIntheasublayerandbsub—layerofIPL，axonsofbipolarceils’expandedterminalswereclearlystainedRecoverinpositiveproductswereshownclearlyastwoparallelbandsAf

8、teropticnervetransection(ONl)，thenumberofrecoverinpositivebipolarceilsgraduallydecreased，incontrastthattheirterminalbuRonsgraduallyincreasedandreachedtopeakatday14，thendroppedatday21and28withaLocaldisarrangementWeconclud

9、edthatthisdatamightsuggestthatafteropticnervetransection，retinalbipolarcells，amaerinecellsandRGCsareundergoingchangesofplasticitybomstructurallyandfunctionallyKeywords：opticnervetransection，PKC—alpha，recoverin，terminalbu