1、西南林學(xué)院2005屆碩士研究生學(xué)位論文華山松高結(jié)實無性系選擇與無性繁殖技術(shù)研究StudyonSelectionandAsexualpropagationTechniquesofHighFertilityPinusarmandiiFranchClones張興指導(dǎo)教師：李桐森教授西南林學(xué)院段安安教授西南林學(xué)院學(xué)位授予單位：西南林學(xué)院云南昆明Abstract：Withmorecognitionabouthighnutritioncontent

2、andsanitarianfunctionofthekernelsofPinusarmandiibeingknown，moreandmoreseedswillbedemandedHowevermostoftheexistingPingsarmandiiforesthavinglowreproductivecapacitySOit’SdifficulttosupplyenoughseedsItisnecessarytosearchaway

3、assoonaspossibletosupplylargequantitiesofseedlingspossessinghighfertilityviaselectingfertileclonesfromtheprimaryseedorchardSeedselectionhasbeenexploredpreliminarilyatZixishanclonalseed—orchardinChuxiongcountyYunnanprovin

4、ceforhighfertilityclones，aswellaspropagatingthroughcuttingandembryocultureIntheseedselection，thirteenhighfertihtycloneshavebeenselectedprimarilybyphenotype，alsothegeneralappraisalofthemhavebeenmadeviaavailableseedsquanti

5、tysingletreeandseedsyieldssingletreeEffectofcuttingageandkindsandcongcentrationsofplantgrowthregulators，culturemediaandclonesonrootingwasstudiedbycuttingInthispapereffectofdifferentplantgrowthregulatorscombinationonadven

6、titiousbudsdifferentiation，androotingalsoWasstudiedthroughembryocultureAndtheresultswereasfollows：①CloneNo44，42and56havekeptsuccessivelyhighyieldofconeintwoyears，andtheaveragenumbersofsingletreewereinfrontItindicatedthat

7、thesecloneshadhighfertility②Beingappraisedandselectedbyessentialseedsquantityofsingletreeandseedsyieldsofsingletree，cloneNo44，42，98and43excelledotherclonesinseedsyieldsofsingletree，andcloneNo44，84and25exceedothersinaspec

8、tsofsupplyingmoreavailableseedsW1lilecloneNo44，84。25，43and42hadpreferablecharacteroftheavailableseedsquantityofsingletreeandseedsyieldsofsingletree⑧Sandisthemostproperculturemediumforrootingamongvermiculite，forestsoil，an

9、dsand；AndIAAisthemostproperplantgrowthregulators麗mtheconcentrationat100ppmamongusedplantgrowthregulators④ThecuttingstudyofthethirteenclonesshowedthattherewasremarkabledifferenceofrootingrateamongclonesCoefficientofextend

10、ingpropagationwasthemostimportantfactorwhicheffectedthecapabilityofpropagation，andcloneNo59，8，42，101，25，84and44andwerebetter⑤Highconcentrationofthesugarwasbadtostartupculture；theculturemediumsupplementwithBAl5m礦5％sugarNA