1、西南交通大學碩士學位論文耐鎘酵母的篩選鑒定及其富鎘機制的初步研究姓名：王小波申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：李學如20121026AbstractCdhasbeenwidelyusedinchemicalindustryelectronicsindustrynuclearindustryandotherfields，everyyearalargenumberofCdwasreleasedintoenvironmen

2、tthroughexhaustgas，wastewaterandwasteresidueintheworldCdisnotessentialelementsforbiologicalgrowthanddevelopmentprocess，consideredasthehighlytoxicheavymetals，CdalongwithHg，As，CrPbarecalled”SINSofelement”ofenvironmentalpol

3、lutionOncegettingintothebody，Cdwillbeaccumulated，causingkindsofphysiologicaldiseases，suchasprostatecancerkidneycanceranditaiitaidisease，etcRecently”cadmiumrice”andotherCdpollutionincidentsreportsalsobroughtalotofnegmivee

4、ffectstoourpeople’SlifeTherefore，thetreatmentofcadmiumpollutionisextremelyurgentBecauseofhighcost，easytocausethesecondpollutionandotherdisadvantages，thetraditionalphysicalandchemicalcadmiumpollutioncontrolmethodshavebeen

5、limitedWithsimpleoperation，highefficiencywiderangeandotheradvantages，themicrobialtreatmentmethodismoreandmorepopularAndyeastisgoodmaterialforthemicrobialtreatmentmethodoftheenvironmentbioremediation2cadmiumresistingstrai

6、nswerescreenedfromthe16strainsofyeastsuppliedbyourlab，namedasY7andY8，whichwereidentifiedasKluyveromycesmarxianusandRhodotorulamucilaginosathroughconventionalclassificationmethodsincludingcolonycharacteristics，cellmorphol

7、ogyphysiologicalandbiochemicalproperties，aswellas26SrDNAsequenceanalysisBothY7andY8hadhighresistanceandeffectualadsorptiontocadmium，evenundercadmiumconcentrationof50mg／L，theirOD600valuewereabove50，morethan70％ofthecadmium

8、inthemediumwereremovedaRer24hoursincubation，andtheoptimalconditionswerepHof60，tempe：ratureof30。Cinoculationvolune5％，250mLErlenmeyerflaskwith30mLfementationmediumandNaClof3％Thedistributionanalysisofcadmiumindi毹rent丘action