1、河南大學(xué)碩士學(xué)位論文擬南芥Atg58基因在受精過(guò)程中的功能分析姓名：焦嬋申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：細(xì)胞生物學(xué)指導(dǎo)教師：苗琛201106IXABSTRACTDoublefertilizationisapeculiarphenomenonfangiosperm.Aftermaturesintheantherpollengerminatesgetsintotheblastula.Thentwospermsanutritionalcellrele

2、asesfrompollentubes.Twospermsmergeoospherepolarnucleusrespectively.That’stheangiosperm.Inthisprocesspollengerminationenlongationareveryimptant.Therearemanyfactsaffectingthedevelopmentofthepollentheapicalgrowthofthepollen

3、tubefexampleATPaseCa2pHsoon.RSSG58wasobtainedfromscreeningricepollencellstwocellsubtractivelibrary.Thegeneisstronglyexpressedinthespermcells.Atg58ofArabidopsisthalianaisthehomologousgeneofRSSG58.WealreadyshowedthatAtg58m

4、ainlyexpressedinthematurepollen.WeconstructedAtg58::GFPvecttoseetheexpressionofthisgeneinthedevelopmentgeneratedsometransgenicplantexpressingthevectfoundthatAtg58localizesincytoplasm.TheexpressionofAtg58iselevatedsignifi

5、cantlywhenthepollensgerminate.Thegenealsoexpressedinpollentube.ByusingtheRNAimutantwemeasuredthedifferenceofmanyparametersbetweentheRNAiwildtypeplantsinvestigatedtheactivityofATPaseofpollensstainingofthepollenswithDAPIch

6、angesinCa2fluescenceofpollens.WefoundthatthedeclinedexpressionofAtg58causethereductionoftheactivityofATPaseintheprocessofthepollenmaturedelayinthedivisionphasefromtwonucleustothreenucleusreductionofthecontentofCa2inthema

7、turepollens.MeovertheCa2polarcontributionduringelongationofpollentubesisalsoaffectedinAtg58RNAimutantplants.TheseresultssuggensthatAtg58affectsnotonlytheprocessofthepollendevelopmentbutalsotheelongationofpollentube.KEYWD