1、南京林業(yè)大學(xué)博士學(xué)位論文百合屬部分種親緣關(guān)系與岷江百合群體遺傳結(jié)構(gòu)研究姓名：吳祝華申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：林木遺傳育種指導(dǎo)教師：施季森20080701ResearchonGeneticRelationshipConservationGeneticsofLiliumSpeciesandofLregaleAbstractChinaisnaturedistributionalcenterofLiliumspecies，Researcheson

2、wildresourcesareanimportantbasetobreedneweultivars(1)Thedistribution，ecologyenvironmentof19Liliumspeciesand2unknowntypesinmostofSouthwardofChinaandnortheastofChinawereinvestigatedandcollectedLbrowniivarviridulum，三1ancifo

3、lium三tsingtauenseandLspeciosumvargloriosoides，Ldauricum，LconcolorLdistichum，Lpumilum，L。browniivarviridulum，LbrowniiL1ancifolium三1eucanthum，Lbrownii，Lbrowniivarviridulum，￡regale，工sargentiae，￡slllphurreum。LhenryiLdavidiiLr

4、osthorniiLduchartreiL10phophorumand三sp1，三sp2wereinvestigatedandcollected(2)Geneticrelationshipwasanalyzedbasedonthedataofpollenmorphology，ISSRmolecularmarker，ITSsequencesofnrDNAandtrnLFsequencesofcpDNAfromeachspeciesTher

5、esultshowthattherewerecloselyrelationshipamongLrosthornii，L1ancifolium，三sp2，三davidii，Lconcolor；amongLbrowniivarviridulum，￡brownii，Lsp1；betweenLTsingtauenseandLdistichumandamong三dauricum，Lsulphureum，L1eucanthum，LtogaIeLsa

6、rgentiae(3)ThegeneticdiversityandspatialautocorrelationofgeneticstructureofLregalewasanalyzedbaseonISSRmolecularmarkerAtthespecieslevel，theproportionofpolymorphiclociwas977％，theeffectivenumberofallele(Ne)was19944，Shannon

7、diversityindex(I)was03339Atthepopulationlevel，theaverageexpectedheterozygosiswas0664Shannondiversityindex(I)was02720Theyshowedthatgeneticdiversityof三regalewashighTheGstof三regalewas016l9，(1isp一坳叩)／Hspwas01537，theresultbyA

8、MOVAanalysisindicatedthatthevariationwithinpopulationaccount838％andvariationamongpopulationsaccountedforl1849％，andthegeneflOWwas2588TheydemonstratedthattherelationshipofpopulationswascloserGenedifirerentiationwasacutewit

9、hinpopulationThespatialautoeorrelationofgeneticstructureof4populationswereanalyzedTheMoran’S1eorrelogramrevealednosignificantspatialstructureinthe4populationsItindicatedthatgeneticvariationsofthemostpolymorphiclociinthes

10、epopulationswererandomlydistributedHowever，thereweregapsatthedistanceof3～4m，5～6and8～10m，andtherewereintrusionatalittlepolymorphiclociKeywords：Lilium；Geneticrelationship；Pollenmorphologycharacters；ISSRmolecularmarker；ITSs