1、黑龍江大學碩士學位論文香鱗毛蕨中生物活性物質(zhì)的篩選姓名：張瑩瑩申請學位級別：碩士專業(yè)：微生物學指導教師：張彥龍20080510黑龍江大學碩士學位論文AbstractDryopterisfragrans(L)Scott，adeciduousperennialherbinDryopterisfamilyofDryopterisgenus，growsinslopinglandwithstonesofhighcoldareaandforesto

2、rInthecracksofmagmanearthevolcano，anddistributesinHeilongjiang，Jilin，andHebeiprovinceInHeiiongiiang，itmainlydistributesinWudalianchi，Bai—ka’shoumountainofTa—hetownalpineregionofDa_baiMountainandthenorthareaofXiaoxing—a11

3、1ingMountainDryopterisfragrans(L)Scon，withstrongbiologicalactivityhavealonghistoryandhasbeenwidelyusedTofindthenewactivecompound，thispapercarriedoutaseriesofsystematicresearchesAftertheethanolextractsofDryopterisfragrans

4、(L)ScottwereabsorbedinmacroporousabsorptionresinAB一8，30％ethanoleluate，70％ethanoleluateand90％ethanoleluateweregatheredThroughscreeningtheanti—tumoractivitiesoftwotumorscellsandtheimmunoregulationactivitiesofTlymphaticcell

5、s，30％ethanoleluatewasdeterminedastheanalgesticeffectivepartsOnthebasisofformereffectivepartsdetermined，30％ethanolelutewasisolatedbySilicagel，ODSchromatographyandHPLCActivetrackwasfullyusedduringtheisolatingprocess，whichp

6、erformedtheantitumoractivescreeningtoBel一7402，A549andtheimmunoregulationactivescreeningtoTlymphaticcellsrespectivelyDuringtheresearch，twoantitumoractiveeluatesandtwoimmunoregulmionactiveeluateswerefoundMoreovertencompoun

7、dswerecollectedbymeansoffurtherisolationtoelualeBesidesstructureidentificationoftheeluatesWasdonethroughH—NMR，CNMR，HMBCandDEPTcombingESI—MSaswellasthecomparisonbasedontherelatedliteraturedatasWhenthecompoundwasat5斗g／mL，c

8、ompoundnumberthreehadfairlystronginhibitedeffectivepartstotwokindsoftumorcells，whilecompoudnumbertenandfivealongwithConAhadpositiveeffectsonTlymphaticcells，whichindicatedthattheyhadfineimmunoregulationeffectivepartsAsawh

9、ole，thispaperresearchedonactivity—guidedisolationofchemicalcomponentsfromDryopter&fragrans(L)ScottandacquiredthreeeffectiveleadingcompoundsThebioactivitiesofthethreecompoundswereconfirmedforthefirsttime，whichmadeafoundat