1、東北農(nóng)業(yè)大學(xué)博士學(xué)位論文玉米抗矮花葉病遺傳分析及數(shù)量性狀基因定位姓名：王振華申請學(xué)位級別：博士專業(yè)：作物遺傳育種指導(dǎo)教師：楊慶凱2002.6.1墜：里：望!型竺些蘭!墜盟型唑竺璺壘鯉竺!壁型望呈!竺生AbstractSugarcaneMosaicVhus(SCMV)isoneofthemostimportantdiseasesofInaizeinChirnDevelopmentandcultivationofresistanthybri

2、dsaret11eeffectiveslrategytoconlrolSCMV(1)Sixtyelitemaize(ZeamaysL1inbredlineswereevaluatedforreactiontoSCMVwithartificialinoculationin1999and2000Theresultsshowedthat9lines(K22，CN962，P138，Qi318，Zhongzi01，Qi319，etc)werehi

3、ghlyresistanttoSCMVand9(X178，Huobai，K12，Huangza04etc)wereresistantTheclusteranalysisbvSSRmarkersshowedthat47inbredscouldbeclassifiedinto7distinetgroupswithseveralsubgroupsFifteenresistantinbredswerefoundin4sources(A，B，E，

4、G)，respectivelyGroupsEandG11wereidentifiedastwoimportantgroupsforSCMVresistancecN962剮K22couldbcusedtoimprove444andDan340forSCMVresistance，respeetively(2)Of18C環(huán)n吖Tand12domesticpopulations，Pooll9，SynC3，SynC4，Suwanl，Pob46an

5、dPob101showedmoderatelyresistancetoSCMVOtherswerea11susceptible(3)TheinheritanceofresistancetoSCMVwasstudiedatseedlingandadultstagesinprogeniesderivedfromfourcrossesbetweentworesistantlinesHuangza04orP“05，andtwosusceptib

6、lesYel07orMol711他resultsshowedthatadditiveanddominantgeneeffectsaswelIasgeneintemctionscouldbefoundintheinheritanceofresistancetoSCMVandgenecitectswerevariableindifierentcrossesTheheritabilitiesforSCMVresistancewereestim

7、atedhighPa405andHuangza04wereestimatedtocontainatIeastoneandtworesistantgenestoSCMv_respectively(4)negeneticlinkagemapswereconstructedwith89SSRmarkersbasedonmaizepopulationconsistingof184F2individualsfromthecrossYel07(su

8、sⅨ!ptible)Huangza04(resistant)，covering1543cMontotaltenchromosomeswithanaverageintervallengthof173cMWiththemethodofcompositeintervalmapping，threeQTLconferringresistancetoSCMVwereidentifiedonchromosomes3，6，and10inF23andF2

9、4populations，respectivelywhiletwomoreQTLonchromosomesland5werefoandinF24population，respectivelySignificantdominantoroverdominanteffectsfor0TLonchromosomes1and5werefound，andadditiveorpartialdominantetfectsweredeteetedfor0

10、TLonchromosomes3。6and10AllQTLcouldexplain34O％and695％ofphenotypicvarianeeinF2：3andF2：4populations，respectivelyTwoQTLonchromosomes3and10wereinferedasmajorones(5)nlenumberofQTLdetectedforresistancetoSCMVandgeneactionwereliR

11、ledifferentwhilethemajorQTLwerethesameatfourgrowthstagesThreeQTLconferringresistancetoSCMVwereidentifiedonchromosomes3，5，and10atseedlingstage；fourQTLidentifiedonchromosomes3，5，6，10砒elongationandanthesisstage；andfiveQTLid

12、entifiedonchromosomes1，3，5，6，10atfillingstage，respectivelySignificantnegativeandadditiveorpartialdominanteffectswerefoundamongQTLdetectedatfourstagesofplantdevelopmentDoctorateeandidate：W_ngZhenhuaProfessioii：Geneticsand