1、分類號(hào)S813.3學(xué)校代碼10129UDC636學(xué)號(hào)2009201008內(nèi)蒙古絨山羊BMP家族和Noggin基因多態(tài)性與絨毛性狀相關(guān)性分析AssociationofBMPGeneFamilyNogginGenesPolymphismwithWoolTraitsofInnerMongoliaCashmereGoat申請(qǐng)人：牧仁學(xué)科門類：農(nóng)學(xué)學(xué)科專業(yè)：動(dòng)物遺傳育種與繁殖研究方向：絨山羊遺傳育種指導(dǎo)教師：李金泉教授張文廣教授論文提交日期：二〇

2、一二年六月AssociationofBMPGeneFamilyNogginGenesPolymphismwithwooltraitsofInnerMongoliaCashmereGoatAbstractThisstudyinvestigated158innerMongoliacashmeregoatsdesigned17pairsofprimersanalysedthepolymphismofBMP2、BMP4、BMP7nogginge

3、nespromoterexonregionsusingPCRSSCPtechnologyfurthermecrelatedanalysisfibertraitpolymphicregion.TheaimistoexpletheBMP2、BMP4、BMP7nogginfthefibertraitofinnerMongoliacashmeregoatsprovidefinnerMongoliacashmeregoatsbreeding.Th

4、eresultsarefollow:Wedon’tfindthedeterminationofthesequencepolymphismafterdetectionBMP2geneexon123respectively.Wedon’tfindthedeterminationofthesequencepolymphismafterdetectionBMP4promoterregion.BMP4genethreeexonpolymphism

5、detectiondiscoveryofwhichthethirdexonthreegenotypesAABBABtypeBBgenotypecomparedwithAAexon3881bpattheC→Tmutationthesitesofnoncodingregion.CrelationanalysisshowedthatthereisnocrelationfthemutationpointtheInnerMongoliacashm

6、eregoatscashmerethickcashmereyieldwoolfiberlengthfinenessofwoolcashmerelengthcashmerefineness(P0.05).BMP7geneexons2347respectivelypolymphismdetectionexonpolymphismwasdetectedin347whichinthedetectionofexon2thatthefragment

7、containingthemutationmutationsinthegroundsofthesubstitutionofG→Athesiteslocatedinexon2after14bpattheintron.ThetwogenotypesCCCDareobtainedbygenotyping.CrelationanalysisshowedthatthereisnocrelationftheInnerMongoliacashmere

8、goatscashmerethicknesscashmereyieldwoolfiberlengthfinenessofwoolcashmerelengthcashmerefineness(P0.05).NoggingeneexonregionpolymphismdetectionfoundoutsidetheInnerMongoliacashmeregoatsinexonthreegenotypesEEgenotypetheFFEFt