1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文飼用芽孢桿菌對(duì)雞腸道發(fā)育和黏膜免疫調(diào)節(jié)作用的研究姓名：張相偉申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：龍良啟趙京楊20080601華中農(nóng)、叱大學(xué)2008屆碩士學(xué)位論文ABSTRACTBacillusspphadcharacteristicofstrongantireversionforce，producingnutritionsubstancesandregulatingbalanceofintestinalm

2、icroecologyTheaimofresearchwastoinvestigatetheeffectofBacillussppusedinfeedstuffonincreasingweightandintestinalmucosalstructuresandtoexploretheeffectofBacillusspponchicken’SintestinalmucosalimmunityinacuteheatstressThere

3、sultsindicatedasfollow：IAccordingtoqualitativeanalysisofproductiveenzyme，Y10，Y17orY18hadcapabilityofproductiveenzymeexceptY4Resultsindicated：Y10，Y17orY18producedproteaseactivitywere35048U／mL，44689U／mL，21205U／mLinorderbya

4、pplyingFolincolouration2TodeterminedrugresistanceofbacillusstrainsaccordingtodrugsensitiveslipagardiffusionmethodResultsindicated：FourbacillusstrainsreflectedsensitivetoFQNS，Aminoglycosides，MIsdrugandSOonButYI7showeddrug

5、tolerationtotetracyclines，otherdrugsshowedvariabilityinthedifferentstrains3FeedingexperimentWasappliedtoobserveeffectsofBacillusasfeedadditiveonchicken’SincreasingweightTheaverageofweightofY17groupincreasedsignificantlyc

6、omparedtocontrolgroupat7d(P005)TheaverageofweightinY10grouporY18groupincreasedsignificantlycomparedtocontrolgroupat21d儼005)，theaverageofweightinY17groupincreasedextremelysignificantlycomparedtocontrolgroupat21d(尸0o1)Ther

7、esultssuggestedthateffectofBacillussppongrowthpromotiondependedonBacillussppstrainsandhadtimeadditiveeffect4HEstainingwasappliedtoanalyzeeffectsofBacillusasfeedadditiveonchicken’Sintestinalmucosalstructuresinthirtyfiveda

8、yBacillussppusedinfeedstuffpromotedtheheightofintestinevillusandWCvalues，especiallytheheightofintestinevillusandV／Cvaluesincreasedsignificantly(PO05orPOooinY10grouporY17groupcomparedtocontrolgroup；Bacillussppusedinfeedst

9、uffinfluencedtheheightofjejunumvilluscomparedtoothersmallintestine；V／CvaluesintheintestinaltractweredecreasedfromcranialtocaudalgraduallyinallgroupsTheresultssuggestedthatBacillussppusedinfeedstuffpromotedchicken’Sdigest

10、iveandabsorptivefunctionandraisedgrowthperformancethroughimprovingintestinalepithelialstructure5HEstainingmethodandSPimmunohistochemicaltechniquewasappliedtoanalyzeeffectsofBacillusasfeedadditiveonchicken’Sintestinalmuco