1、分類號(hào)Q75學(xué)校代碼10129UDC579學(xué)號(hào)2011211051纖維素降解微生物的富集、分離及相關(guān)酶制劑的初步開發(fā)應(yīng)用纖維素降解微生物的富集、分離及其降解特性分析EnrichmentIsolationacterizationofCellulosedegradationMicroganisms申請(qǐng)人：陳勿力吉瑪學(xué)位類別：工程碩士領(lǐng)域：生物工程指導(dǎo)教師：馮福應(yīng)教授論文提交日期：二〇一三年六月EnrichmentIsolationacter

2、izationofCellulosedegradationMicroganismsAbstractWiththerapiddevelopmentofsocialeconomytherapidincreaseofpopulationenvironmentalpollutionenergycrisisarebecomingsomeseriousproblem.Soseekinganeffectivemethodbecomesmemeimpt

3、ant.Celluloseisthemostabundantcheapestrenewablecarbonsourceinthewld.Itcanbeconvertedtoenergyfoodfeedchemicalrawmaterialsetc.Cellulaseactivityisnothighexcessiveuse.Thatarethemainrestrictingfactsinthewidelyusedcellulose.Th

4、erefetheionofgoodperfmancestableenzymeproductionstrainsisnotonlythebasicwayofreducingthecostofcellulaseproductionbutalsothekeytosolvetheproblemofeffectiveutilizationofcellulose.Inthispaperwestudiedcellulosedegradingmicro

5、ganismsbythepureculture.Theresultsshowedthatstrainsof37wereisolatedbythepureculturemethodunder10℃theresultsshowedthatstrainsof23wereisolatedbythepureculturemethodunder28℃.Thesimilaritiesof16SrRNAgenesequencesoftheisolate

6、swereanalyzed.Theresultsindicatedthatthebacterialgroupscomposedof.StrainCB04outofthesestrainshasthehighestrelativeenzymeactivityunder28℃.Itis114.600Uml.ThroughtheoptimizationoftestbestproducingcellulaseofstrainCB04wasdec

7、ided.Theincubationtimeis4dayspHvalueis7thenitrogensourceisyeastextract.KeyWds:LowtemperatureRoomtemperatureSoilDegradingcelluloseBacteriaPurecultureRelativeenzymeactivityDirectedby:Prof.FENGFuyingApplicantfMasterDegree:C