1、 A Dissertation Submitted to Huazhong University of Science and Technology for the Degree of Doctor of Medicine The Nano-based Immunotherapy and Chemotherapy for the Treatment of an Advanced Melanoma Candidate : Yao

2、 Lu Major : Pharmacology Supervisor : Guangya Xiang Huazhong University of Science & Technology Wuhan 430074, P. R. China Jan 7, 2016 目 錄 主要中英文縮略詞表 ...........................................................

3、........................................... 1 摘 要 ............................................................................................................................ 5 Abstract ..............................

4、.............................................................................................. 8 第一部分 姜黃素納米膠束與納米疫苗聯合在晚期惡性黑色素瘤中的作用 ........... 11 1.1 前言 ....................................................................

5、................................................ 11 1.2 實驗材料和方法 ................................................................................................ 17 1.3 實驗結果 ...............................................

6、............................................................. 31 1.4 討論 .................................................................................................................... 43 第二部分 納米化療藥物順鉑引導的原位抗原產生在晚期腫瘤模

7、型中的應用 ....... 48 2.1 前言 .................................................................................................................... 48 2.2 實驗材料與方法 ................................................................

8、................................ 51 2.3 實驗結果 ............................................................................................................ 55 2.4 討論 ........................................................

9、............................................................ 65 第三部分 結論 ............................................................................................................... 68 參考文獻 ............................

10、............................................................................................ 69 綜 述 .....................................................................................................................

11、....... 76 致 謝 ............................................................................................................................ 98 附 錄 ...................................................................