1、分類號：R733密級：不保密UDC：610學校代碼：11065碩士學位論文CXCL12CXCR4CXCL12CXCR4軸在臍血間充質干細胞影響白血病細胞增殖和軸在臍血間充質干細胞影響白血病細胞增殖和凋亡中作用的研究凋亡中作用的研究李明穎指導教師趙春亭教授學科專業(yè)名稱內科學（血液病學）論文答辯日期2017年5月26日ⅡTheeffectofCXCL12CXCR4signalaxisontheproliferationapoptosisof

2、leukemiccellsculturedwithhumanumbilicalcdbloodderivedmesenchymalstemcellsAbstractObjective:ToinvestigatetheroleofCXCL12CXCR4signalaxistheeffectsofGCSFAMD3100ontheleukemiccellstheirmicroenvironmentinthecoculturemodelofHUC

3、MSCswithHL60(acutemyeloidleukemiacellline)cellsJurkat(acutelymphoblasticleukemiacellline)cells.Methods:HL60cellsJurkatcellswerecoculturedwithhumanumbilicalcdbloodmesenchymalstemcells(HUCMSCs)themodelwasmanagedwithGCSFAMD

4、3100theircombinationrespectively.CellviabilitycellcycleweremeasuredbyCellCountingKit8(CCK8).CellapoptosiswasassessedbyflowcytometryusingtheAnnexinVApoptosisKitaswellasthecellcycleanalysis.TheexpressionofsurfaceCXCR4prote

5、intotalCXCR4proteinofleukemiccellsweredetectedbyflowcytometryWesternblotrespectively.Results:HUCMSCscoulddecreasetheviabilityofHL60cellsJurkatcellsthepercentageofapoptoticcellstheycanalsoincreasethenumberofquiescentcells

6、whileGCSFAMD3100couldfurtherreducetheproliferationofHL60cellsJurkatcellsinHUCMSCcoculturemodeldestructedtheantiapoptoticeffectofHUCMSConHL60cellsJurkatcellsthecombinationoftwodrugsresultedinasynergisticeffect.GCSFcouldre

7、ducetheexpressionofsurfaceCXCR4proteintotalCXCR4proteininleukemiccellswhileAMD3100couldonlydecreasetheexpressionofCXCR4onthesurfaceofleukemiamembranehavingnoeffectontheexpressionofCXCR4proteinincytoplasm.Conclusion:Human

8、umbilicalcdbloodmesenchymalstemcellscaninhibittheproliferationapoptosisofacuteleukemiacellsincreasethenumberofG0G1phasecellsinleukemiccells.AMD3100candecreasetheexpressionofsurfaceCXCR4proteininleukemiacellsGCSFcandecrea

9、setotalCXCR4proteinaswellasmembraneCXCR4proteinexpression.BothofthemcanblocktheCXCL12CXCR4signalaxisweakeningtherelationshipbetweenleukemiacellsmicroenvironment.onthebasicofHUCMSCinfluencedleukemiacellsgrowthproliferatio