1、福建農(nóng)林大學(xué)碩士學(xué)位論文辣椒CabZIP蛋白RNAi載體的構(gòu)建及其遺傳轉(zhuǎn)化研究姓名：官德義申請學(xué)位級別：碩士專業(yè)：農(nóng)業(yè)推廣農(nóng)學(xué)指導(dǎo)教師：何水林祁建民20091101福建農(nóng)林大學(xué)農(nóng)業(yè)推廣碩士專業(yè)學(xué)位論文AbstractCapsicumannuumLisoneofthemostimportantvegetablesalloverchina，andalsoanimportantexportingcropsinChinaAvastofreduc

2、tioninitsyieldisusuallycausedbycontinuousexposuretOvariousstressesincludingabioticsuchasdrought，abnormaltemperature，andbioticstressessuchasdiseasesMolecularmechanismofpepperstressesstressresistanceisimportantforitsgenedc

3、improvementPreviousstudiessuggestedthattranscriptionfactorsplaygreatrolesintheadaptationofplantstOvariousstresses，andbasicleucinezipperisoneofthemostconservativetranscriptionfactorsandexistwidelyineukaryoteMembersofthebZ

4、IPfamilyhavebeenidentifiedinthegenomeofArabidopsis，riceandsoybeanandplayimportantrolesintheregulationofplantgrowthanddevelopment，transductionoflightsignal，defensereaction，responseofbioticandabioticstressesaswellasABAmedi

5、atedsignaltransductionSofarlittleisknownabouttherolesofmembersofbZlPfamilyinpepperanditispresumedthatbZIPsmayalsoplayimportantrolesinenvironmentaladaptationofPepPerandthefunctionalstudyofbZIPsisimportantformolecularmecha

6、nismelucidationandefficientgeneticimprovementofpepperstressesresistanceTheRNAivectorsof3bZIPcandidategenesisolatedfromCapsicumannuumeDNAlibraryofleaveschallengedwithpathogeninfectioninthepreviousstudieswereconstructedint

7、hisstudybyusingthe5’or3’untranslatedregionsofthefulllengthcDNAsofthe3candidategenesandthegatewaycompatiblepHELLSGATE12vectorThe3RNAivectorswerefurthertransformedintothegenomeofalocalpeppervarietyp120byAgrobacteriummediat

8、edmethodandthe89，12clonesofTOregeneratedKanresistantplantsofthe3RNAivectorsWasacquired，and45，8correspondingtransgenicplantswereverifiedbyPCRamplificationprimedbythespecificprimersofthecorresponding3bZIPregions，andthecorr

9、espondingT1lineswerealsoacquiredbyselfpollinationTheresultsofthisstudylaidanimportantbaseforthefurtherfunctionalanalysisofthe3candidategenesbyRNAianalysisKeywords：Capsicumannuum；bZIP；transcriptionfactor；RNAinterfere；gene