1、山東農(nóng)業(yè)大學(xué)碩士學(xué)位論文產(chǎn)朊假絲酵母利用有機酸及其發(fā)酵蘋果渣生產(chǎn)菌體蛋白的研究姓名：高衛(wèi)衛(wèi)申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：杜金華20090614產(chǎn)朊假絲酵母利用有機酸及其發(fā)酵蘋果渣生產(chǎn)菌體蛋白的研究AbstractApplepomace，whichcontainstheabundanceofnutrientssuchasvitamins，organicacids，sugarsetc，istheby—productafterap

2、pleprocessingSofar,theapplepomacehasnotbeenutilizedeffectivelyItcancontaminatetheenvironmentinsomeoccasionsThedevelopmentofappleindustryhasthereafterbeenaffectedTherefore，producingmicrobialproteinusingapplepomacefermente

3、dbymicroorganismsisareasonableandeconomicwaytocomprehensivelyutilizetheapplepomaceApplepomacehasexcessiveorganicacidslikeL—malate，lactate，acetateandcitrateSomeapplepomacesaretoosourtouseasanimalfeedsdirectlyThisproblemca

4、nbepotentiallysolvediftheorganicacidsintheapplepomacecanbeutilizedasthesourceofcarbonbysomemicroorganismsduringthefermentationHere，theabilitiesofCandidautilistoutilizeextracellularorganicacidsastheirsourceofcarbonwerefir

5、stlystudiedThen，theeffectofsomeparameterssuchasinoculumsize，ratioofmaterialtowater,ureaaddition，thedurationoffermentationandthefermentationtemperature，werestudiedtoimprovetheproteincontentsinfermentedapplepomaceproductsB

6、asedonthepreliminarysingle-factorexperiments，fourvariables(inoculumsize，ratioofmaterialtowater,ureaadditionandthedurationoffermentation)atfourlevelshavebeenoptimizedfurtherbyorthogonalexperimenttoobtaintheoptimumparamete

7、rstoproducesolidfermentedapplepomacemicrobialproteinsThemainresearchresultswereasfollows：1SomeextracellularorganicacidssuchasL—malate，lactate，succinateandcitratecouldbeutilizedassinglecarbonsourcestosupportthegrowthofCan

8、didautilis，whilesomeotherorganicacidssuchasoxalate，acetateandfumaratecouldnotbeutilized2Glucosewasutilizedinprioritywhenitexistedwithsuccinate，citrateorLmalateinthemediumNodecreaseinthecontentsoftheseorganicacidswasobser

9、vedwhentheglucosewasdepletedThisindicatedtheuptakeoftheseorganicacidswasinhibitedbytheexistingglucoseinthemedium3WhenLmalate，lactate，succinate，andcitratesimultaneouslyexistedinthemedium，lactateWasmosteasilyusedbyCandidau