1、浙江大學生命科學學院博士學位論文鹽環(huán)境細菌群落結構分析和多相分類學研究姓名：吳月紅申請學位級別：博士專業(yè)：微生物學指導教師：吳敏20090501浙江大學博士學位論文鹽環(huán)境細菌群落結構分析和多相分類學研究AbstractHalophilicmicroorganismsaremicrobesinhabitedindiversesalineenvironmentsAccordingtotheiradaptationtosaltsuppleme

2、ntedinmedia，thesemicrobesaredividedintohalophilicandhalotolerantgroupsThehalophiliemicroorganismscangrowuptothehighsaltconcentrations，suchassaltlakes，sodalakes，solarsaltems，hypersalinesoils，marineenvironments，etc，andthey

3、oftenhavestrongimpactontheecosystemsinwhichthethriveTl艙propertyofmicrobialadaptationtohighsaltenvironmentsismainlyrelationwithenzymesandotherbio-activesubstanceMuchinterestinhalophilicadaptationhasbeensupportedbythesearc

4、hforlifeontheplanetsandtheapplicationsinbiotcchnologySoitisverysignificantnotonlyintheorybutalsoinpracticetostudyhalophilesMyresearchisbasedonourlab’Sworksuchassamplescollectionfromhi【曲saline，microorganismscultivationand

5、relatedmoleculartechnologyAyakekumuLakelocatedinXinjiangregion，theestuaryoftheYangtzeRiveroftheEastChinaseaandtheChinanoduleprovinceinEastPacificoceanwhichwerecollectedfromthreeenvironmentecosystemswereinvestigatedCultiv

6、ation-independentapproachesbasedonrRNAcloningandsequencingwereappliedtosurveymicrobialdiversityinnaturalecosystemsTheidentificationandbiodiversityofculturablemicroorganismswereperformedbyusingthemoleculartechniquesubsequ

7、entlyBasedontheanalysisof16SrDNAgenesequenceanalysisandphenotypecharacteristics，thedatabaseofhalophileswasestablishedandenrichedInthisstudyusingmolecularbiologymethods，construct16SrDNA，napA，n／rSbiomarkergenesclonelibrari

8、esattheestuaryoftheYangtzeRiveroftheEastChinaseaTotal366cloneswereanalysisand256clonesweresequencedandphylogeneticanalysedBacteriabiodiversitywasveryrich，mainlycomposedby7-Proteobacteria，a-Proteobacteria，Acidobacteria，6一

9、ProteobacteriaandBacteroidetesItindicatemicrobialcommunitystructureandtheirpossiblegeochemicalprocessInaccordancewiththeprincipleofpolyphasictaxonomy,asystematicstudyhadbeenconductedonthetaxonomyofeightisolatesSixnovelmo

10、deratehalophilicbacteriaspecies(Halomonascaseinilytica,Halomonassalifodinae,MarinobacterPelagius,Pseudidiomarinamaritima，Pseudidioraarinadon#aiensis,Hbriohangzhouensis)aswellasonehalotorleranthalophilicbacteriaspecies(Mi