1、ADissertationSubmittedinPartialFulfillmentoftheRequirementsftheDegreeofMasterTheProtectiveEffectofPuerhBlackTeaExtractonSubchronicallyExposedQuinocetoneinducedOxidativeDNADamageofKidneyinSDRatsCidate:XiaoLuoMaj:FoodDrugS

2、afetyManagementSupervis:Pro.LiegangLiuPro.XiaoliLiuHuazhongUniversityofScienceTechnologyWuhanHubei430074P.R.ChinaDecember2011目錄摘要...........................................................................................

3、.................................1ABSTRACT...................................................................................................................3前言............................................................

4、................................................................51材料和方法.................................................................................................................81.1主要試劑和儀器.........................

5、.........................................................................81.2受試物.................................................................................................................111.3試驗(yàn)動(dòng)物..................

6、...........................................................................................112實(shí)驗(yàn)方法....................................................................................................................122.1動(dòng)

7、物分組及飼養(yǎng).................................................................................................122.2組織病理檢查....................................................................................................122.3血

8、清生化檢測(cè)....................................................................................................132.4ROS檢測(cè).........................................................................................................

9、...132.5腎臟勻漿氧化抗氧化指標(biāo)檢測(cè)........................................................................132.6腎分離細(xì)胞DNA損傷檢測(cè).............................................................................162.7SD大鼠尿液8OHdG檢測(cè)...............

10、...............................................................182.8統(tǒng)計(jì)分析............................................................................................................213研究結(jié)果.................................

11、..................................................................................223.1一般毒理學(xué)指標(biāo)觀(guān)察........................................................................................223.2ROS檢測(cè)..........................

12、..................................................................................253.3腎臟勻漿線(xiàn)粒體生化檢測(cè)................................................................................273.4腎分離細(xì)胞DNA損傷檢測(cè).........................

13、....................................................283.5SD大鼠尿液8OHdG檢測(cè)..............................................................................314討論...................................................................

14、.......................................................33參考文獻(xiàn)......................................................................................................................37綜述.....................................

15、.........................................................................................42附錄..............................................................................................................................5