1、南京財(cái)經(jīng)大學(xué)碩士學(xué)位論文高溫菜籽粕蛋白質(zhì)的提取及其酶法改性姓名：劉勝申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：食品科學(xué)指導(dǎo)教師：王素雅201001IIIABSTRACTThetechnicalparametersofexatractingproteinfromindustricalrapeseedmealbymeansofcombinationmethodproteinasemethodwereestablishedrespectively.Thestr

2、ucturefunctionalpropertiesofextractingrapeseedproteinwerestudied.Finallythefunctionalpropertiesofrapeseedproteinthatwaslimitedhydrolysisbyneutralproteinasealkalineproteinasewereinestigatedtoo.Theoptimumextractingrapeseed

3、proteintechnicalparameterofcombinationmethodwereasfollows:enzymedoes2.5%ratioofmaterialtosolvent1:20pH7.050reactiontime3haqueousalkalipH10.0552hratioofmaterialtosolvent℃℃1:12theextractionrateofrapeseedproteinwas71.63%.Th

4、eextractionwasprecipitatedatisoelectricpointsthendecolizedby70%ethanol.Thepurityofisolatedrapeseedproteinwas78.44%contentsoftanninphyticglucosinolatewere1.14%1.05%0.17mggrespectively.Glucosinolatecontentislowerthanedible

5、grade(Theglucosinolatecontentislowerthan0.4mgginfood).Theoptimumparametersofneutralproteinasemethodwereasfollows:enzymedoes1%ratioofmaterialtosolvent1:20pH7.555℃extracted3htheextractionrateofrapeseedproteinwas76.16%.Thep

6、urityofrapeseedproteinwas67.84%contentsoftanninphyticglucosinolatewere2.04%、1.65%、1.78mggrespectively.Theproductisonlysuitableffeedadditive.Tostudymolecularcomposesstructureofbothextractedrapeseedproteintheresultshowed:a

7、minoacidcompositionofbothextractedrapeseedproteinweresimliar.Rapeseedproteinwhichwaspreparedbycombinationmethodhadmuchmelargemolecularweightofproteinitsrelativemolecularmassdistributedat279000Da12600Da4200Datheelutionare

8、aaccountedf38.96%17.76%41.63%respectively.Rapeseedproteinbyproteinasemethodhadmuchmesmallmolecularweightofproteinitsrelativemolecularmassmainlydistributedat288000Da62000Da3005Datheelutionareaaccountedf5.13%、7.18%、85.62%r

9、espectively.circulardichroism(abbreviationCD)spectrumindicateddifferencesbetweenextractedproteinbycombationmethodpreparedproteinbyproteinasemethodonsecondarystructuretherewereαhelixβsheetβturnromcoil51.27%1.22%21.68%25.8