1、華中科技大學碩士學位論文靶向納米造影劑C225USPIO標記裸鼠鼻咽癌移植瘤MR成像研究姓名：錢瑩申請學位級別：碩士專業(yè)：腫瘤學指導教師：胡國清201105華中科技大學碩士學位論文2TheresearchonMRIofcontrastagentnanoparticlesC225USPIOmarkertargetingtothenasopharyngealcarcinomaxenograftinnudemiceCidate:QianYin

2、gSupervis:Prof.HuGuoqingAbstractObjectivePreparethecontrastagentnanoparticlesC225USPIOtargetingtotheepidermalgrowthfactreceptobservethevalueofMRIofnasopharyngealcarcinomaxenograftinnudemicemarkedbythisagent.MethodsCombin

3、ingC225(Cetuximab)withUSPIOthroughEDCwhichaimedattargetedcontrastagentnanoparticles(C225USPIO)thediameteroftheagentwasmeasuredbyGranulometerAtomicFceMicroscope(AFM)TheEGFRhighexpressionofnasopharyngealcarcinomaSUNE1lines

4、weretransplantedintothethighventralsubcutaneousintherighthindlegof12femalenudemiceWhenthediametersoftransplantedtumgrowupto5mmdividethemromlyintotwogroups.Sixfexperimentalgroup(C225USPIO)theotherfcontrolgroup(USPIO).C225

5、USPIO(1000molFekg)USPIO(1000molFekg)wereseparatelyinjectedintonudemicethroughcaudalveinrespectivelyscannedby4.7T30cmsmallanimalsMRT2WIImagingafter082472hourwhichaimedatanalyzingthesignalstrengthofdifferenttimepointstheex

6、istenceofcontrastagentnanoparticlesinthetumtissueswhichweredetectedbyPrussianBlueStaining.ResultsPSAAFMshowedthatthediametersofC225USPIOwereabout4550nm.MRIshowedthatafterinjectingC225USPIOintheexperimentalgroupT2valueofx