1、復旦大學碩士學位論文多巴胺系統(tǒng)基因和帕金森病的相關性研究姓名：徐玲申請學位級別：碩士專業(yè)：遺傳學指導教師：任大明20020528摘要AssociationAnalysisbetweenDopaminemetabolismsystemgenesandParkinson’Sdisease隊bstraeOParkinson’Sdisease(eB)isamukigenicdiseasewithcomplexpathogenicity，char

2、acterizedbydenaturalizationanddecreaseofthedopaminergicneuronandLewybodiesItiswidelyacceptedthatPDonsetisduetotheCO—functionofenvironmentandgeneticsfactorsTheDopaminemetabolismsystemisverycomplexandinvolvesmanyenzymes，an

3、ddysfunctionofanyenzymewilldisturbthebalanceofdopaminesystemInthisstudyweexaminedthepolymorphismsoffivedopaminesystemgenes(includingCOMTDBH，DATMAOAandMAOB)，in154unrelatedidiopathicPDpatientsand231agematchedhealthycontrol

4、sthroughthemethodsofrestrictivefragmentlengthpolymorphism(RFLP)andampli句fragmentlengthpolymorphism(AFLP)OddsratioWascalculatedtoinvestigatetheassociationbetweenthesegenesandPDTheresultswereasfo1lows：1，ThegenotypeA2／A2and

5、alleleA2ofDBHgeneTaqIpolymorphismweresignificantlyassociatedwithPD2TherewerenosignificantdifferencesofthedistributionofCOMTgeneG／ApolymorphismbetweenPDandcontrolsevenafterthesubjectswereseparatedbyageof603Nosignificantdi

6、fferencesexistedinthedistributionofMAOAgeneEcoRVpolymorphismbetweenPDandcontrols，eitherinsubgroupaged604AfterstriatificationbytheDBHTaqIgenotypes，therewerestillnosignificantdifferenceofCOMTMAOApolymorphicdistributionbetw

7、eenPDandcontrols5Thealleles一186bpand≤170bpofthe(GT)nmicrosatellitepolymorphismintheinon2ofMAOBgeneweresignificantlyassociatedwithPD6TherewerenodistributiondifferencesoftheDATgene40bpVNTRpolymorphismbetweenPDandcontrolsRe