1、東北農(nóng)業(yè)大學碩士學位論文黃瓜植株高度遺傳分析及分子標記姓名：辛明申請學位級別：碩士專業(yè)：蔬菜學指導教師：秦智偉20070620MolecularmarkcrandGeneticAnalysisofPlantHeightincucumberAbstractCucumberisoneofimportantvegetables，andcanbeeateninfourseasonsandhaveimportantrelationtopeople

2、lifeButcultivatedvarietiesofcacumberarealltrailingplantsnowadaysWiththeimprovementofllyingJevelthemethodofdwarfcucumberhayedbeellmoreandmorestudiadandspresdedtoincreaseyieldTOsolvethecontradictionofpopulationgrowthandcro

3、planda他asincreasinglyreductionandtoincreaseeconomicretumsofcultivantedcucumberweshouldselectandcultivatetidealheightvarietyascultivarswithoutsupportorcultivarswithoneofrmechanizationharvestFirst，wecareaboutphysiognomicqu

4、ality，whichisdirectfactorofpurchasingdesireApplingt0thegeneticanalysissoftwareofQGAStationI0theparentsandFIF2of4x4diallelcrossesamomgseveraldeterminateanddwarfphenotypeofcucumberweleusedinthisstudyandcarriedoutthegenetic

5、analysisforcacumberlengthThisstudyusedF2populationderivedfromcombinationstoconstructmolecularmarkerlinkagemapandidentifrlawofinheritanceHeattoleranceoftheparentsandF2populationwereidantifyedInadditionamolecularmarkerlink

6、agemapwasconstructedwithSSRmarkerandQTLananlysiswasperformedwithcompositeintervalmapping(CIM)Themainresultsa“笛foIlowe：1ThegeneticanalysisofplantheighthasbeencarriedoutforbYusingadditivdominancccpisteticemedic(ADAA)Result

7、indicatesthatplantheightaccordswithADAAmedicandtheplantheightwasmainlycontrolledbyindictiveandalsocontrolledbyinteractionofadditivdominanceandadditivepistationTheanalysisofinteractionofadditiveandenvironmenteffectsindica

8、testhatplantheightWasefleetedmainlybyinteractionofadditivandenvironment，whiletheinteractionofdominanceandenvironmentandthejnteractionofepistatic：eandenvironmentrarelyeffected，andtheeitherbroadheritabilityornarrowheritabi

9、lityofcucumberareheigh。2Byvarianceanalysisofplantheight，intemodenumberandintemodeheight，thisstudyfoundintheidenticalckcumstance，thepSantisheigbertheintemodeIongger，andviceversa3Inthisstudythefactorswhichaffected013theSSR

10、PCRresultsofCucumberWerestudiedincludingtheconcentrationofdNTPprimerMg“，DNAtemplateTaqDNApolymeraseTheresultshowedthattheoptimalreactionsystemwasasfollows：the25rtlreactionsystemcontaineddNTP250pmol／L，PrimerO5pmol／L、Mg”15