1、華中科技大學(xué)碩士學(xué)位論文血管緊張素Ⅱ的1型受體在體外培養(yǎng)的牛眼小梁細(xì)胞的表達(dá)姓名：秦文申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：眼科學(xué)指導(dǎo)教師：熊新春20090501華 中 科 技 大 學(xué) 碩 士 學(xué) 位 論 文 華 中 科 技 大 學(xué) 碩 士 學(xué) 位 論 文 Expression of angiotensin II type 1 recepter angiotensin II type 1 recepter in cultured bovine tr

2、abecular meshwork cells Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology Postgraduate: Qin Wen Tutor: Xiong Xinchun Professor Abstract Objective：To

3、 determine whether bovine trabecular meshwork cells have the expression of angiotensin II type 1 recepter(AT1R) in vitro, and to interpret the role of angiotensin II type 1 recepter in the the circulation of aqueous humo

4、r and regulation of ocular tension. Methods：The bovine trabecular meshwork cells were cultured in vitro and were passaged to generate the third passage, then the cells were transfered or cultured onto steriled cover glas

5、s. Angiotension II type 1 recepter expression were detected by immunohistochemistry. Reverse transcriptase-polymerase chain reaction(RT-PCR) was applied to determine the existence of angiotensin II type 1 recepter mRNA i

6、n bovine trabecular meshwork cells. Results：Cultured cells showed the specific characteristics of bovine trabecular meshwork cells. Bovine trabecular meshwork cells showed yellow-brown staining in the membrane and cyt

7、oplasm, indicating a positive expression of angiotensin II type 1 recepter, whereby no positive staining was found in negative control. Furthermore, mRNA encoding of angiotensin II type 1 recepter was found in bovine tra

8、becular meshwork cells. Conclusion：Bovine trabecular cells have the expression of angiotensin II type 1 recepter, which may have important role in aqueous outflow and the development of glaucoma. Keywords：bovine trabecul