1、東南大學(xué)碩士學(xué)位論文孕婦外周血漿中胎兒DNA的基因芯片檢測研究姓名：晏菱申請學(xué)位級別：碩士專業(yè)：婦產(chǎn)科學(xué)指導(dǎo)教師：蔣犁20070419東南大學(xué)碩士學(xué)位論文AbstractTitle：Study01“ItheDetectionofFetalDNAinMaternalPlasmaUsingDNAMicroarrayPostgraduate：YANLingTutor：ⅡANG“NameoftheUniversity：SoutheastUniv

2、ersityobjeetive：ToevaluatethemethodofmicroarrayinnoninvasivedeterminationoffetalYchromosomespecialsequencesinfasttrimestersearchappropriatemethodsforextractionpurificationandPCRamplificationoffetalDNAformicroarrayMethods

3、：Methods：1FetalDNAswereextractedfromtheplasmaofpregnantwomenanddividedintoformaldehydetreatedgroupandnonformaldehydetreatedgroup(controlgroup)ThenSRYsequencewasamplifiedandSRYpositivecaseswereselect，thenSRYsequenceswerea

4、mplified，paetinactingaspositivecontr01TheirPCRresultswereanalyzedbyGelProAnalyzersoftware，thedifferenceofthetwogroupswerecomparetodemonstratetheeffectoftheformaldehydetreatedmethodintheincreasingoftheproportionofthefetal

5、DNAinbloodplasma2(1)AdultnlaleDNAwasdilutedFadllally，andDYSwasamplifiedusingthemethodsofcommonPeRandmicroemulsionsPCRrepectivelytocomparethedetectionlimitofthetwomethods(2)AsthetemplateDNAwasextractedfrommalecarrierswhic

6、hdeterminedbyamrfioticfluiddetectionThensixYchromosomespecialsequenceswereamplifiedbythemethodsofcomlnonmultiplexPCRandmicroemulsionsmultiplexPeRtocomparethedifferenceofthetwomethods3Mieroarraycouplewithmieroemulsionsmul

7、tiplexPCRwasusedtodeterminefetalgenderinfirsttrimesterbydetectionsixYchromosomespecialsequencesandanintemalcon舡olgeneAndinordertoevaluatethediagnosisefficiancyresultswereconlparedtofetalgenderatbirthtoealculatothesensiti

8、vityandthespecificityResults：111∞SRY／13actinratioofformaldehydetreatedgroupWaSO77士o13andtheSRY／pacthlratioofnonformaldehyde—treatedgroupwasO22士007，theaveragerelativeconcentrationoffetalDNAinmaternalbloodwashigherinformal