1、青島大學碩士學位論文黑色素瘤抗原基因在胃癌組織中表達的研究姓名：關向宏申請學位級別：碩士專業(yè)：病原生物學指導教師：羅兵2002.4.10英文摘要STUDY0NEXPRESSIONOFMELANoMAANTIGENGENESINGASTIUCCARCINoMABSTRACTobjectiveAsahumantumor—specificantigenthatcanberecognizedbyCTL，MAGEgenefamilyhashigh

2、expressionrateinvarioustumortissuesanditsproductofgeneexpressionhasgoodtumorspecificitywhichmakesitahotspotofresearchatpresentWestudiedtheexpressionofMAGE—l，一2and3genesin30samplesofhumangastriccarcinomasandtheassociation

3、betweentheexpressionsanddifferentiation，aswellastheexpressionsandclinicalstage，tryingtolaythetheoreticalandexperimentalfoundationforusingthesegenesproductsasthevaccinetoperformimmunotherapyonpatientsofgastriccarcinomaMet

4、hodsTheexpressionofMAGE12and一3genesin30gastriccarcinomasspecimensand30correspondingadjacentnormalgastrictissuespecimenswasdetectedbyareversetranscriptase———polymerasechainreaction(RTPCR)TheresultswereanalyzedaccordingtOc

5、linicopathologicfactorsofthetumorThe6samplesofRTPCRproductsweresequencedResultsMAGEgenesfamilyhashighexpressionrateingastriccarcinoma20(667％1ofthe30gastriccarcinomasspecimensexpressedatleastoneoftheMAGEmRNA，ofwhich14spec

6、imensexpressedMAGElmRNA，9specimensexpressedMAGE2mRNAand11specimensexpressedMAGE3mRNA，withthepositiveratesbeing467％，30O％，and367％respectivelyTherewasnosignificantstatisticdifferencebetweentheexpressionofthesethreeMAGEgenes

7、rx!=1796，P=0407005)TherewasnoexpressionofthesethreeMAGEgenesintheadjacenttissueofgastriccarcinomaThereWasmarkedsignificantstatisticdiffefencebetweentheexpressionratesofthethreeMAGEgenesintissueofcarcinomaandthoseintheadi