1、福建農(nóng)林大學(xué)碩士學(xué)位論文蠟蚧輪枝菌液體發(fā)酵條件的研究姓名：殷華申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：關(guān)雄謝明2002.5.1福建農(nóng)林大學(xué)碩士學(xué)位論文AbstractAbstractVerticillumlecaniiisawell—documentedentomopathogen，andwashighlyeffectiveincontrolofhomoptexaninsectsIthasbeenobservedtocau

2、seepizooficsincertainconditionsThisfimgushasbeenusedastheactiveingredientincommercialmycoinsecticidesforbiologicalcontrolofaphidsandwhitefliesItsproductshadbeenonsaleinforeigncountries，butinourcountry，therelatedresearche

3、sareinthepr／marystage，andit’scrucialtodevelopmentThisresearchaimedtofindoptimizedcultureconditionsofliquidfermentation，andthemaincontentsareasfollows：1Basingonthecriterionsoffastgerminationandcolonygrowth，andofratherhigh

4、conidiumproductioninsolidandliquidculturemedium，CA一19hasbeenselectedfortheresearchofliquidfermentationfrom8typesofV／ecanii2Throughsomeparameterstestedintheexperimentsofflaskliquidculture，andtheorthonormalexperimentoffour

5、factorsthree1evelsoptimizedsubstrateandflaskculturecondhionswereobtmned(2％yeast，25％glucose；50mlsubsratecapacityin250miflask1％inoculation)3Obtainedliquidfermentationtechniqursconditionsarebenefittoindustrialfermentationpr

6、oductionDO(dissolutionoxygen)≥10～15％；OriginalpHcontrolintherangeof55and75；510％inoculation；Adoptingdifferentculturetemperturesffrom25℃to23。C)4Underthatfavouredconditionssomeparametersaremensuratedin5Lfermentationerliquidc