1、東北農(nóng)業(yè)大學博士學位論文小鼠乳腺組織鐘基因的篩選與鑒定姓名：齊幫若申請學位級別：博士專業(yè)：生物學；生物化學與分子生物學指導教師：李慶章20120531AbstractScreeningandIdentificationofClockGenesinMouseMammaryGlandABSTRACTBiologicalrhythmisthebasicphenomenonoflifeBiologicalclocksplayanimportan

2、troletegulateorganismadapttotheenvironmentinavarietyofgenes，differentcellsanddifferentorganizationsWhenwereseachthelifephenomenmustconcemcircadiaIlrhythemsincetheroleofthecircadianisSOextensiveandimportantReseachthenetwo

3、rkofclockgenesandtheregulationmechanismofcircadianclocktomammaryglandisveryimportantScreeneddifferentlyexpressedgenesamongdiffenentstagesanddifferenttimeofmammaryglandinthisstudyAnalysisgenesfunctionthatdifferentlyexpres

4、sedInthisexperimentmousegenomicgenechip430ofAffymetrixwasusedtoscreendifferentiallyexpressedgenesindifferentdevelopmentperiodsofmousemammaryglandAndgeneexpressionprofilewasobtainedbycomparisonofgeneexpressionbetweenlacta

5、tionandinnovationAtthess／netime，theresultswereverifiedbyfluorescencequantitativeRTPCRInthisexperiment，965differentiallyexpressedgeneswerescreenedoutbycomparisonofdayandnightinlactation，and629geneswerehighexpressionand336

6、geneswerelowexpressionatdaytime5317differentiallyexpressedgeneswerescreenedoutbycomparisonoflactationandinvolution，and2137geneswerehighexpressionand3180geneswerelowexpressionatlactationTheresultsshowedthatdifferentiallye

7、xpressedgenesinlactationstage，weremainlyfocusedonthesynthesisandsecretionofmilkDayandnightdifferentialexpressiongenesfunction，weremainlyconcentratedinthebiologicalrhythmsScreeneddifferentiallyexpressedgenesinthePathwayan

8、alysis，andrelativelyactivesignalingPathwayAccordingtothemicroarrayarrayresults，weusequantitativePCRmethodtostudyPerl，Per2，Bmall，CrylandcyegeneswhichdifferentlyexpressedindifferentdevelopmentstageanddifferenttimepointTher

9、esultsshowedthatthePer2andBmallhavelargerimpactOnmammaryglanddevelopmentPer2exhibitedaincreasedexpressioninmammaryglandduringadolescenceandmiddlepregnancy伊005)RhythmanalysisofClockgeneinSCNandmammaryglandshowthatatLDcond

10、itionsthephaseofClockinmammaryglandhaveareverserelationshiptoSCN，andatDDconditionsthephaseofClockinmammaryglandhavethesamerelationshiptoSCNAnalyzingtheClockandPerlgenesfunctionwithRNAinterferencetechnologyCaseinandlactos