1、華南農業(yè)大學碩士學位論文狗牙根ASR基因的克隆和功能分析姓名：付有亮申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：郭振飛201206CloningandAnalysisofCdASRGenefromBermudagrassFuYouliang(CollegeofLifeSciences，SouthChinaAgriculturalUniversityGuangzhou510642，China)Abstract：Bermudagr

2、ass(CynodondactylonL、isoneofthemostimportantWarlTl—seasongrasses，whichisextensivelyusedinturfestablishmentandlivestockherbageforitspotentialdroughtandheattoleranceandlowmaintenancerequirementsinwarmtemperateregionsofthew

3、orldAbscisicacid(ABA)isanimportantsignalfortriggeringplantresponsestoadverseenvironmentalconditionsduringvegetativegrowth，andABAcoordinatesmanyofthesestressresponses，includingtheimmediatestomatalclosure，osmolyteaccumulat

4、ionandtheinductionofthesynthesisofstress—relatedproteins，suchaslateembryogenesisabundantandheatshockproteins，reactiveoxygenscavengers，etcAABA—inducedeDNAlibrarywasconstructedusingSolexasystemfrombermudagrassHighthroughpu

5、ttagbasedanalysisusingSolexasequencingwasplatfromedonbermudagrasstranscriptomeofA2(shorttermtreatment)，A3(10ngtermtreatment)，andA1(contr01)7040genesareannotatedbymappingtothedatabasesequencesWeusedFDR(falsediscoveryrates

6、)andtheabsolutevalueofll092Ratio[=1asathresholdtojudgethestatisticalsignificanceofgeneexpressionFromthefourdatasets，wefoundplentyofgenesdifferentiallyexpressedbetweentwolibrariesOfthese，179wereupregulmedand97weredown—reg

7、ulatedinexpressionTheexpressionlevelsof38differentiallyexpressedgeneswereconfirmedbyRTPCRFinallyASR(Abscisicacid，StressandRipening)genewasselectedTheASRproteinsarehighlycharged，hydrophilicandlowmolecularweightproteinstha

8、tarewidelypresentintheplantkingdom，exceptintheBrassicaceaefamilyASRshavebeendescribedasaccumulatingtohighlevelsduringplantdevelopmentalprocesses，suchasfruitripening，pollenmaturationandGlcmetabolism，andinresponsetodiverse