1、西北大學(xué)碩士學(xué)位論文AEC柱前衍生化寡糖鏈的HPLC分離及LCESIMS分析姓名：郭文申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：中藥學(xué)指導(dǎo)教師：黃琳娟20100613HPLCandLCESIMSanalysisofoligosaccharideschainbypre—columnderivatizationwith3amino一9一ethylcarbazoleAbstractInthispaperweused3amino一9一ethylcarbazole

2、(AEC)asaderivatizingreagentforderivatizationanalysischitooligosaccharidesandNglycanchainofglycoproteinsbasedonanalysisAEC—labeledmonosaccharidesThederivativeswereseparatedbyreversephaseHPLCandidentifiedbyLCESIMSFinallywe

3、haveobtainedtheseresultsasfollows：1InthispaperweuseAECforderivatizationanalysischitooligosaccharidesThederivativeswereseparatedbyreverse—phaseHPLCandidentifiedbyESIMSandonlineLCESIMSWeoptimizedtheHPLCconditionsofLCESI—MS

4、analysis，ThecolumnusedWasfilledwithRPC18packing(5#m，250minx46mm)ThemobilephaseWascomprisedof10mmol／Lammomumacetate，pH45(solventA)，andacetonitrile(solventB)，theAEC—labeledchitooligosaccharideswereelutedusingagradientprogr

5、amasfollowing：from90％Ato75％Afor80minataflowrateof02mL／minTheAEC1abeledchitooligosaccharidesWasdetectedbyUVat254nnqToachieveasimultaneousseparationandstructureidentifiedthedifferentdegreeofpolymerizationofoligosaccharides

6、2WefurtherexpandtheapplicationofAEC，useitasaderivatizingreagentforderivatizationanalysisNglycanchainofribonucleaseBandhenovalbuminEstablishedthemethodforderivatizationanalysisNglycanchainsseparatedbyreverse—phaseHPLCandi

7、dentifiedbyESI—MSandonlineLCESIMSAtfirst，weusePNGaseFenzymeteleasedNglycanchainofglycoproteinandisolationandpurificationitCompletethisstep，weuseAECasaderivatizingreagentforderivatizationanalysisNglycanchainofribonuclease