1、西北大學碩士學位論文稀缺藥用植物小蔓長春花產(chǎn)活性成分內(nèi)生真菌的研究姓名：孫宇宏申請學位級別：碩士專業(yè)：微生物學指導教師：殷紅20090601Abstract’Tenstrainsofendophyticfungiwereisolatedfromthestems，rootsandleavesofararemedicinalplantVincaminorLinnAccordingtothecharacteristiccolorreactio

2、nsofalkaloids，onestrain，XM—J2withtheabilitytoproducealkaloidswerescreenedoutinthepreliminaryselectionThenthestrainwasfurtherselectedbythinlayerchromatography(TLC)AndtheexperimentalresultsshowedthatXM—J2fermentationbrothe

3、xtracthasthesameRfvalueinTLCanalysisasthatofstandardvincamineBasedontheaboveexperiments，theprocedureofvincamineextractionwasoptimizedThroughsilicagelcolumnchromatography(CC)，50％methanolisservedaseluentagentAfurtherUVspec

4、trometryandhigh—performanceliquidchromatography(HPLC)showedthatoneingredientoftheextractfromthestrainXM—J2hadasimilarabsorptioncurveinthewavelengthrangeof200500nmandthesameretentiontimeasvincamineThestructureofthesamplew

5、asconfirmedbyusingtheIR‘H—NMR、HHCOSYTheresultsshowedthatthesampleshouldcontainedthebioactiveingredientvincaminelikeitshostplantVincaminorUsingthebiomassandvincamineyieldasindexes，anoptimumliquidmediumpotato—sucrosemedium

6、Wasselectedout；themaximumyieldofvincamineinthismediumWas064mg‘L～Then，bychangingdifferentcarbonsource，nitrogensourceandculturetemperature，optimalcultureconditionswereselectedThesuitableconditionsforbothmycelialgrowthandvi

7、ncamineproductionare“potatosucrosemedium謝thsucrose，peptone，andthetemperatureabout28oC，respectivelyThefermentationconditionsofXMJ2werefurtherpotimizedbytheorthogonalexperimentofL16(4’)Theresultsshowedthatsucrose50g／L，pept

8、one1g／L，pHabout70andthetemperatureabout28。C，theliquidmediumquantityabout8590ml／250mlweretheoptimumconditionsforitsmycelialgrowthandweresuitableforvincamineproductionThestrainXMJ2WasclassifiedprimarilyXMJ2shouldbeastrainb