1、內(nèi)蒙古農(nóng)業(yè)大學(xué)研究生學(xué)位論文獨創(chuàng)聲明本人申明所呈交的學(xué)位論文是我本人在導(dǎo)師指導(dǎo)下進行的研究工作及取得的研究成果。據(jù)我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包括其他人已經(jīng)發(fā)表或撰寫過的研究成果，也不包括為獲得我?；蚱渌逃龣C構(gòu)的學(xué)位或證書而使用過的材料，與我一同工作的同志對本研究所做的任何貢獻均已在論文中作了明確的說明并表示謝意。申請學(xué)位論文與資料若有不實之處，本人承擔(dān)一切相關(guān)責(zé)任。論文作者簽名：4交叢叢日期：’盤壘!塑=(!

2、望內(nèi)蒙古農(nóng)業(yè)大學(xué)研究生學(xué)位論文版權(quán)使用授權(quán)書本人完全了解內(nèi)蒙古農(nóng)業(yè)大學(xué)有關(guān)保護知識產(chǎn)權(quán)的規(guī)定，即：研究生在攻讀學(xué)位期間論文工作的知識產(chǎn)權(quán)單位屬內(nèi)蒙古農(nóng)業(yè)大學(xué)。本人保證畢業(yè)離校后，發(fā)表論文或使用論文工作成果時署名單位為內(nèi)蒙古農(nóng)業(yè)大學(xué)，且導(dǎo)師為通訊作者，通訊作者單位亦署名為內(nèi)蒙古農(nóng)業(yè)大學(xué)。學(xué)校有權(quán)保留并向國家有關(guān)部門或機構(gòu)送交論文的復(fù)印件和電子文檔，允許論文被查閱和借閱。學(xué)?？梢怨紝W(xué)位論文的全部或部分內(nèi)容(保密內(nèi)容除外)，采用影印、縮印或

3、其他手段保存論文。論文作者簽名：《蠡毯盤厶一指導(dǎo)教師馘：2駐!奴日期：兇墼么!!oPreliminaryAssemblyofMongolianSheepGenomeandTranscriptomeAnalysesofcoldtolenceinMongoliansheepAbstractMongolianSheep，beingknowntOitshardinessandgoodmeatqualityistheancientsheepbre

4、edinChinaThegenomecontainsagreatdealofpreciousgeneticresourcesInthisstudywesequenedthewholegenomeoftheMongoliansheepusingIlluminaHiseq2000sequencingtechnologyplatformandthewhole—genomeshotgunsequencingapproach，inordertop

5、rovidethebaseoffilteringthegeneswithfinepropertiessuchasanticoldnessdiseaseresistanceere。Theresultshowedthatthegenomesizewasestimatedin29lGbAssemblycontigN50lengthiS201KbseaabldN50l朋gthiS27313KbAverageGC％ofthesequencewas

6、42％，andtheGCaveragedepthiSmoTethan40incoveragedistributionRepeatsequcnceisI0lGbaccountingfor3471％andtransposableelementsiSaccountingfor90％inrepeatsequenceUsingthegenestructureandgenefunctionannotation，21，704geneswerepred

7、icted，thefunctionof20，600geneswerenoted，thesizeofthenon—codingRNAaccountsfor0OI5％ofthegenomeTherearel7，015genefamiliesinMongoliansheepBasedonthegenomeresearch，weusedaCGHsystemtoidentifytheCNVsinthesheepgenomeandconstruct

8、edtheCNVsmapjnMongoliansheep，Kazakhsheep，Tibetansheep，HusheepandDorpersheep5lCNVRsweretestedcontaining24gains，22lossesand5gain—lossesTheHBBgenewasamplifiedinMongoliansheepandTibetansheepwhichmaybeattributedtoadaptability

9、inlowoxygenandhighaltitudeenvironmentForthecold—resistaneeofMongoliansheep，DoperSheepasreference，wesequencedthetranscriptomeoffat，muscles，adrenalglandsandhypothalamusinthecoldconditionusinglllumina／Solexatechnologyandsel

10、ected529，75，268andl61differentlyexpressedgenesAccordingtodifferentlyexpressedgenesof伍ttissuebyKEGGsignalingpathwaytheresultsweremainlyrelatedwithfatcelldifferentiation，lipidsynthesis，energymetabolismI33up—regulatedgenesw

11、erefilteredinfat，inwhichRXR，F(xiàn)ATP，AOP7involvedinPPARcellsignalingwiththefunctionofacceleratingpreadipocytedifferentiationandtransportinglong—chainfattyacid；GB氏T3ALDHinvolvedinglycerolipidmetabolismsignalingpathwayandregul

12、atethesynthesisoftriglyceride；1RSlandLEPinvolvedintheregulationofenergymetabolism；theribosome28Ssubunitandl8Ssubunitinvolvedinproteins審nthesis10differentlyexpressedgeneswhichplayedanimportantroleinlipidsynthesisandenergy

13、metabolismwereselectedtobeverifiedusingqPCRTheresultswereequivalenttORNA—seqIncoldconditions，theleveloflipidsynthesisandenergymetabolismandproteinsynthesisinfattissueofMongoliansheepwashigherthanDoperSheep，whichshowedMon