1、中國(guó)農(nóng)業(yè)大學(xué)碩士學(xué)位論文擬南芥中AtMEK4和AtMEK5的啟動(dòng)子組織特異性及其對(duì)脅迫響應(yīng)的初步研究姓名：周天虹申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：任東濤劉國(guó)琴20060601Abs仃actTheMAPK(mitogen—activatedProteinkinase)oaScadehasbeenconsideredasoneofthehighlyconservedsignalingpathwaysineukaryoti

2、ccells，anditplaysveryimportantrolesincellgrowth，division，differentiation，developmentandsignalingregulationofstressresponsesMAPKincludesthreecomponentswhichcanbeactivatedinseries：MAPKKK(MAPkinasekinasekinase)MAPKK(MAPkina

3、sekinase)andMAPK，AtierexocellularsignalsaredeliveredtoMAPKcascadesthroughtheirreceptors，MAPKKKcanbeactivatedbymultiplephosphorylationmechanisms，thenitcanactivateMAPKKandMAPKthroughMAPKKKMAPKK)MAPKpathwayActivatedMAPKcanb

4、eeitherdirectlymovedtonucleartoactivatespecifictranscriptionfactorsorstayincytoplasmtophosphorylatecbaoskeletonassociatedProteinsorotherProteinsandenzymes，tomakespecificphysiologicalresponsestoexocellularstressesAtMEK4an

5、dAtMEK5arereposedastwoMAPKKgenesinArabidopsisTheyarehighlyconservedandfunctionallyredundantinsignalingregulationsofcelldeath，ethylenesynthesisandresponsestopathogenelicitorsHoweverlittleisknownabouttheirrelationshipanddi

6、fferenceintheirexpressionandfunctionSowetrytostudytheirlocalizationinArabidopsisinordertoanswerthesequestionsThePromotersofAtMEK4andAtMEK5wereclonedbyPCRandthenfusedwithgus(gglucuronidase)reportergeneinvectorpBll21ThePr0

7、4GUSandPr05一GUSweretransformedtoArabidopsisbyfloraldippingGUSassayofthetransgenicplantsshowsthatPr04一GUSactivityishighinrosetteleaves，baseofpetiole，partsofleafprimordium，andmatureflower(petal，stigma，filamentandanther)Pr0

8、5一GUSactivityishighinroottip，stele，andmatureflower(stigmaandanther)Aftertreatingthetransgenicplantswithcoldness，NaCIandpathogen(PseudomonasSyringaepvtomatoDC3000)，onlythepathogendecreasedGUSactivityinordertostudythetissu

9、elocalizationofAtMEK4andAtMEK5，wefusedtheirPromoterstogtp(greenfluorescentProtein)invectorpBll21andtransformthemtoArabidopsisTheresultsshowthattheyareexpressedindifferentpartsofroottips：GFP—AtMEK4isobservedinProcambium，b