1、山東大學(xué)碩士學(xué)位論文季也蒙畢赤氏酵母產(chǎn)菊粉酶的研究姓名：王光遠申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：陳冠軍20050516山東大學(xué)碩十學(xué)位論文ResearchontheInulinaseofPichiaguilliermondiiAbstractYeaststrainSDU290wasscreenedfromthecollectionofonrlabfbritshjgharproductionofinulinaseThisstrai

2、nwasidentified越Pichiaguilliermondiibymorphology。physiologyandtheidentifiedsystemofautomaticmicroorganismofBIOLOGTheinfluence011producinginulinasewithdifferentcarbonsouroes，nitrogensources，initialpH，metalchemicalcompoundw

3、asstudied，theresultsindicatedthattheoptimumfermentedmediumwas：inulin25％；yeastextract08％；(N戰(zhàn))2HP0402％；MgS04’7H205mmoVL；pH55Usingtheseoptimalconditions，thisstrainWasfermentedinfermentorandtheenzymeactivityoftheirfermentedl

4、iquidWasmeasured，theresultsshowedthattheenzymeproducingpeakappearedafterbeingeultivatedfor60hoursandthehi曲estfermentedliquidcuzymeaetivityreached30U／m1ThedistributionoftheinulinaseofSDU290Wasstudiedandtheresultsshowedtha

5、ttheinulinasedistributedwithincellWallandeellmainlyandthcrewaslittleinulinasesecretedoutsidetheeellThecomponentsofINUlandDTU2wereisolatedandpurifiedfromcellwallandfermentedliquidrespectivelybyusingion—cxchangechromatogra

6、phy耐tllDEAEscphoraseandexclusioiIchromatography喇tllBioGelP1OOEachcomponentshowedonebandonSDSPAGEandWaSidentifiedtheSalTlecomponentwithmolecularweight84l(D扎theplWasmeasuredtobe43byIEEEnzymeresponseresearchindicatedthatthe

7、optimaltemperatureWas55“CTheactivityandstabilityinpn4565werehighandstableTheinfluenceofmetalion011theenzymeactivitywasstudiedCharacterizationofthereactionproductsWaSdonebythin—layerchromatographytheresultsshowedthatthepr