1、南京林業(yè)大學(xué)碩士學(xué)位論文里氏木霉纖維素酶的分離純化與酶學(xué)性質(zhì)研究姓名：于兆海申請學(xué)位級別：碩士專業(yè)：生物化工指導(dǎo)教師：江華20060601Pu尚cationandCharacterizationofCeHulasesfromtheFungusTdchodermareeseiYuZhaohaiSupervisedbyProfessorJiangHua(ConegeofChemicalEngineering，NanjingForestryU

2、niversity210037)June2006AbstractUsingultrafiltration，cationic(CMSepharoseFDandanionic(DEAESepharoscFF)chromatographyandsizeexclusion(SephadexG100)，sixcdlulolyticenzymeswerepurifiedfromtheculturebrothofTrichodermareesei：t

3、hreeendoglucanases(EGIEGII，EGffl)，twoceUobiohydrolases(CBHI，CBH]I)anda肛gulcosidase(GB)ThesixceHulasesdisplayedonesinglebandonSDSPAGErespectivelyThespecificactivityofEGI正GIIandEGmwitheathoxyinethylceHulosewasestimatedtobe

4、17635IUmg“115396IUmg16422IUmg“1respectivelyForCBHIandCBHII血cspecificactivitywas1686IUmgq482IUmg1respectivelywithmierocelluloseThespecificactivityofthe13gulcosidasewitllsalicinwas31_00IUna91Basicenzymaticcharacteristicofo

5、btainedcellulaseswerestudiedTherelativemolecularweightofEGI，EGII。EGⅢ，CBHI，CBHⅡandGBwas50kD，46kD，25kD，65kD，581kDand75kD，respectivelyKineticsmethodshowedtheKmofEGI，EGII，EGIIIforcarboxymethylcellulosewas670meCmL，846mg／mL，13

6、22mgCmLrespectivelyForCBHIandCBHII，theKmWithmicroceUulosewas137mg／mL’and346mg／mLrespectivelyTheKmofB—gulcosidasewithsalicinwas220mg／mLForEGI，EGII，EGIII，CBHI，CBHⅡandGB，theoptimumtemperturewas55“C，50(2，55“C，60。C，45(2and60。

7、CrespectivelywhiletheiroptimumpHat56，44，50，56，50and50Besides，theeeUulaseshadtherelativelygoodstabilityunder30。C，whileinthetempertureupon80℃theylosttheiractivityquicklyTheceHulaseswererelativelystablewithinpH5ON62EGI和CBHI