1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文嗜水氣單胞菌血清型、膜表面成分和胞外產(chǎn)物特性研究姓名：董傳甫申請學(xué)位級(jí)別：碩士專業(yè)：水產(chǎn)養(yǎng)殖指導(dǎo)教師：林天龍王明學(xué)2002.5.1華中農(nóng)業(yè)大學(xué)2002年碩士學(xué)位論文OSerogrouping，SurfacecomponentsandECPsProfilesofAe_ro衄onashydrophiIaAcollectionofAeromonasstrainsofdifferentoriginswereclassif

2、iedacc。rdingtotheirO—serogroupingantigenBasingontheCHserogroupclassificationresLllLS，Partialcharacterizationofthelipop01ysaccharides(LPSs)，Sarrayprotein(SAP)outermembraneprotein((NP)，andextraeeiiularproducts(ECPs)ofsomea

3、bovebacterialstrainSwereanalysedbyusingimmunological，serologiealmethodsandSI)SPAGEThecorelationbetweenserotypesand1]roteinpatternsoftheseisolateshadbeendiscussed(i)Isolating，identifyingandOserogroupmgofAeromonashydrophil

4、aAero忉onasstrainswhichweremainlyisolatedfromthediseasedeelSand]r／onyxs]nensfsinPujianprovincewerecharacterizedbybothbiochemicalassaysandpolymerasechainreaction(PeR)Amongthesetestedstrains，therewere59Aihia，AAvdrophlstraim

5、s30sobriastrains2Caviestrainsandmorethan10otherAeromonasspp，respectivelyAntiserumraisedagainst4formalinizedbacterialstrainsand9boilingtreatedbacterialstrainswerepreparedinrabbitsAndtheantiserumagainsttheboiiingtreatedbac

6、teriastandedforfivemainOserogroupsUsingtheseantiserumforclassification，44outof131motileAeFomonasbelongedtOfiveOserogroupsbybothtubeagglutinationandmicroplateagglutinationIntheserotvpeO：Ahl0501，mostofthestrainswereFujiani

7、solates，andallisolatesfromtheTrlonyxSIBCBSISresidedinthisserotypeMostofthetypicalAeromonashydrophilaisolateswhichwerenotisolatedfromFujianwereclassifiedinto0：Ah9617However，thebacterialisolatedfromeelsappeareddiverseinthe

8、irserotypeswithoutd∞inantOserotypesThestructureandantigenieityoftheLPSfromdifferentiso]atesofseveralserotypesof月hydrophilawerefurtheranalysedbySDS—PAGEandwesternblotingAndtheresultsdemonstratedthattheLPSwesternblotpatter

9、nswerehighlYaccordantwithagglutinationassay，andmoreaccurateinserotypingthanthatbyagglutinationassayItprovedthatLPSwesternblotpatternWaSanewapproachwhichcouldbeappliedforclassificationofthemotileAeromonasspecies(2)Cell—su

10、rfacecomponentsprofilesofAero呵onashydrophiJaS。mecel卜surfacefeatures，includingautoa991utination(AA)，precipitationafterbei1ing(PAB)andCongo—redadsorptionwereinvestigatedinAeromonasflydrophilawithdifierentOserogroupsNoappar