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1、中國(guó)農(nóng)業(yè)科學(xué)院博士學(xué)位論文陸地棉雙列雜交的遺傳效應(yīng)及表達(dá)譜分析姓名:楊代剛申請(qǐng)學(xué)位級(jí)別:博士專業(yè):作物遺傳育種指導(dǎo)教師:喻樹(shù)迅20110520AbstractCottonisthemostimportantfibercropinChinaandthewodd,anduplandcottonaccountsformorethan95%cottonproductionYangtzefiverbasinYellowriverbasinandX
2、injianga他thethreemajorcottongrowingregionsinChinaStudyofthegeneticeffectsandtheexpressionprofdeofuplandcottoncultivarsfromthethreemaingrowingareasshouldbehelpfulforselectionforthebackboneparents,heterosispredictionandmol
3、ecularbreedingincottonInthisstudy,10representativeuplandcottoncultivarsfromChina‘SthreemajorcoRon—growingregions,usingadiallelmatingdesignmethod,wereusedtoproduce45F1hybridsandtheir45F2generationsHeldexperimentswerecarri
4、edoutin2008,2009and2010,inthreetypicalecologicalregionsforcottonproductioninChinaincludingWangjianginAnhuiProvince,AnyanginHenanProvinceandAksuinXinjiangUyghurAutonomousRegionEightmorphologicalandyieldtraitswer;cinvestig
5、atedandfivefiberqualit),characters嘴surveyedGenemicroarrayanalysiswasconductedusingfloweringbudsatfullbloomstagein2008StatisticalanalysisWasconductedusingthegeneticmodelincludingadditivedominanceepitasisanditsinteractione
6、ffectwithenvironm蚰tconditionanalysismethodQTLNetworkV30Themainresultsaleasfollows:(1)Fortheeightmorphologicalandyieldtraits,themaingeneticeffectsandtheirinteractionwithenvironmentplayaroleatvaryingdegreesAdditiveby—addit
7、iveepistaticeffectsonbollnumberperplantbollweightlintpercentage,seedindexandlintyield,interactionbetweenadditivoadditiveepistaticeffectandenvironmentonlintpercentageandseedindexwerepositivelysignificantGeneralheritabilit
8、yinthenalTOWsense(l芻)forbollweightlintpercentageandseedindexwerehigh,indicatingthatearlygenerationselectionWaSeffectivePlantheight,numberoffruitingbranchesandbollnumberperplantweregreatlyinfluencedbyenvironmentindicating
9、thatdirectselectionislessreliableInteractionheritabilityinthebroadsense(日品)for舭numberoffruitingbranches,bollnumber。seedcottonyieldandlintyieldwererelativelylarge(314%一518%),indicatingthatheterosisCallbeusedinspecificenvi
10、ronmentsParents9018,CCRl41,sGK958and2028callbeusedashybddparentstoconcurrentlyimprovetwoorthreeyieldcomponents(especiallylintpercentage),leadingtosignificantlyincreaselintyieldTheadditiveeffectandadditiveaddtiveepistasis
11、Offlintpercentageandseedindexinparent2028Waslargeandpositivelysignificant,andshouldbeusedinselectionbreedingUsing339215455asparentwasexpectedtoeffectivelyimprovebollweightandthusincreaselintyield(2)Forthefiberqualitytrai
12、ts,boththemaingeneticeffectsandtheirinteractionwiththeenvironmentplayarolebutthedifferencew蠲largerFiberstrengthwagmainlyaffectedbyadditiveeffects,whichcallbegeneticallyimprovedbyconventionalbreedinghGZandgeneralheritabil
13、ityinthebroadsense(n。2;)ofupperhalfmeanlengthwe∞large,andearlygenerationselectioncanalsobeconductedandheterosiscanbeutilizedinspecificenvironmentve|vPofuniformityindex。elongationandmicronairevaluewereabove40%,whichnleans
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