1、華中科技大學碩士學位論文缺氧條件下曲安奈德對Müller細胞表達的水通道蛋白4的影響姓名：曾苗申請學位級別：碩士專業(yè)：眼科學指導教師：程揚20090501華 中 科 技 大 學 碩 士 學 位 論 文華 中 科 技 大 學 碩 士 學 位 論 文 3Effect of Triamcinolone Acetonide on the expression of AQP-4 in Müller cell under hyp

2、oxia Master candidate: Zeng Miao Tutor: Associate Prof. Cheng Yang Abstract Objective Hypoxia is an important cause in various retina diseases,such as retinal edema,diabetic retinopathy,proliferative retinopathy and

3、so on. Clinically,triamcinolone acetonide (TA) was used to resolve retinal edema.In this study we cultured rabbit Müller cells in vitro to investigate the effect of TA on the expression of AQP-4 in Müller cells

4、 under hypoxic conditions. Method The hypoxic model was set up with chemical hypoxic inducer- CoCl2. Cultured rabbit Müller cells were divided into five groups.Group A was negative control group; Group B was culture

5、d under 150umol/L CoCl2 for 24 hours; Group C was cultured under 10mg/L TA for 24 hours and then cultured under 150umol/L CoCl2 for 24 hours; Group D was cultured under 40mg/L TA for 24 hours and then cultured under 150u

6、mol/L CoCl2 for 24 hours; Group E was cultured under 400mg/L TA for 24 hours and then cultured under 150umol/L CoCl2 for 24 hours. The expression of AQP-4 protein in five groups was detected by immunocytochemical method.

7、 The expression of AQP-4 mRNA was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results The expression of AQP-4 protein was increased markedly by the CoCl2, and TA could preve

8、nt it. The inhibition of TA was dose-dependent. AQP-4 expression at transcription was enhanced by the CoCl2 , and decreased by TA. Conclution TA could decrease the expression of AQP-4 in Müller cells under hypoxic