1、東北大學(xué)碩士學(xué)位論文微流控分析系統(tǒng)與傳統(tǒng)毛細(xì)管電泳的初步比較研究姓名：姚波申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：徐淑坤2001.12.1東北大學(xué)碩士學(xué)位論文ABSTRACTFundamentalResearchonComparisonbetweenMicrofluidicChipBasedCESystemandTraditionalCESystemAbstractAmicrofluidicchipbasedCEsystemwithh

2、omemadelaser。inducedfluorescence(LIF)detectionfortwofluorescentreagentsandfourFITClabeledaminoacidswasestablishedFundamentalcharacteristicssuchastheJouleheat，electroosmoticflowandtheseparationefficiencywerestudiedThethes

3、iswasdividedintofivechaptersInthefirstchapterthedevelopmentandachievementofmicrofluidicchiptechnologyWasoverviewedandtheprincipleofamicrofluidicchipbasedCEsystemdrivenbyelectroosmoticflowwithLIFdetectionsystemWaSrepresen

4、tedChapter2gavethefabricationandmaitenanceofsuchachipanddiscussedsomeproblemsworthpayingattentiontoduringtheexperimentInthenextchapteramicrofluidicchipbasedCEsystemdrivenbyelectroosmoticflowwithLIFdetectionWasestablished

5、fortwofluorescentreagentsFluoresceinandCalceinThe“pinch’’injectionmodeandanothermodejustlike‘‘gate”whichwerereportedbeforewereemployedforsampleintroductionTheimageoftwofluorescentreagentsinthechannelswasreal—timemonitore

6、dbychargecoupleddetector(CCD)andtheirphotographswerepresentedThejouleheatandseparationefficiencywasdiscussedinthischapterAfterthat，acomparisonbetweenmicrofluidicchipbasedCEandtraditionalCEsystemonjouleheat，electroosmotic

7、flowandseparationefficiencywasdiscussedinchapter4TheV—Acurves(Ohm’Slawplot)ofmicrochipandCEwerestudiedrespectivelybyalternatingtheconcentrationofborateThecurvestillmaintainedlinearityforchipsystemwhenjouleheatWaSupto56W，